Goat anti-Human Golgi Protein, 58K Polyclonal Antibody | anti-USO1 antibody

Golgi Protein, 58K, NT (FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase)

Cat. Num. AAA613359

• Gene Names: USO1; TAP; VDP; P115
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Golgi Protein; 58K; Polyclonal Antibody; NT (FTCD; LCHC1; formiminotransferase cyclodeaminase; formimidoyltransferase cyclodeaminase); Anti -Golgi Protein; anti-USO1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is expected to recognize both reported isoforms (as represented by NP_006648 and NP_996848). Species Crossreactivity: human, mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-USO1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Dilution: Peptide ELISA: >1:64,000; Western Blot: 0.03-0.5ug/ml. A 55-60kD band observed in Human Liver lysate (calculated MW of 58.9kD according to NP_006648); Immunohistochemistry: 3-5µg/ml In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes.
• Immunogen: SQLVECVPNFSEGKNQ, N Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(MBS613359(3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Chemiluminescence

(MBS613359(0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-USO1 antibody

A58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Product Categories/Family for anti-USO1 antibody

Antibodies; Abs to Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505541
• NCBI GeneID: 8615
• NCBI Accession #: NP_003706.1
• NCBI GenBank Nucleotide #: NM_003715.2
• UniProt Accession #: O60763; Q6PK63; Q86TB8; Q8N592; B2RAQ0
• Molecular Weight: 107,895 Da
• NCBI Official Full Name: general vesicular transport factor p115
• NCBI Official Synonym Full Names: USO1 vesicle transport factor
• NCBI Official Symbol: USO1
• NCBI Official Synonym Symbols: TAP; VDP; P115
• NCBI Protein Information: general vesicular transport factor p115; protein USO1 homolog; vesicle-docking protein; vesicle docking protein p115; transcytosis associated protein; USO1 vesicle docking protein homolog; USO1 homolog, vesicle docking protein
• UniProt Protein Name: General vesicular transport factor p115
• Protein Family: General vesicular transport factor
• UniProt Gene Name: USO1
• UniProt Synonym Gene Names: VDP; TAP
• UniProt Entry Name: USO1_HUMAN

NCBI Description

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq, Jul 2008]

Uniprot Description

TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol

Molecular Function: protein transporter activity

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle