Mouse anti-Human Glypican 3 Monoclonal Antibody | anti-GPC3 antibody
Glypican 3 (Glypiated Proteoglycan 3) (PE)
Dilution: Flow Cytometry: Use 10ul to label 1x10e6 cells
Optimal dilutions to be determined by the reseacher.
Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Uniprot Description
GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq26.1
Cellular Component: extracellular space; proteinaceous extracellular matrix; lysosomal lumen; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane
Molecular Function: heparan sulfate proteoglycan binding; protein binding
Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; pathogenesis; positive regulation of endocytosis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of growth; anterior/posterior axis specification; negative regulation of epithelial cell proliferation; lung development
Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1
Research Articles on GPC3
2. SALL4 is a more sensitive marker than PLAP, AFP, or glypican-3 for extragonadal yolk sac tumors.
3. Data show that mutated GPC3 lacking the GPI anchoring domain (sGPC3) significantly inhibited the in vivo growth, blocked Wnt signaling and Erk1/2 and Akt phosphorylation in tumors.
4. Our data supports the potentially significant diagnostic utility of GPC-3 in fine-needle aspiration biopsies in differentiating primary malignant from benign and preneoplastic liver lesions, and metastatic carcinomas.
5. Report the expression profile of glypican-3 and its relation to macrophage population in human hepatocellular carcinoma.
6. GPC3 expression is correlated with poor prognosis in hepatocellular carcinoma.
7. overexpression of glypican-3 may be related to the development and aggressive behavior of ovarian clear cell adenocarcinoma.
8. Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis.
9. A reliable marker for the detection of hepatocellular carcinoma in liver biopsies.
10. A patient with the Simpson-Golabi-Behmel syndrome displays a loss-of-function point mutation in GPC3 that inhibits the attachment of this proteoglycan to the cell surface.
