Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen H Type 2 Monoclonal Antibody (19-OLE))
Mouse anti-Human Blood Group Antigen H Type 2 Monoclonal Antibody | anti-ABO antibody
Blood Group Antigen H Type 2 (CD173) Mouse Monoclonal Antibody
Gene Names
ABO; GTB; NAGAT; A3GALNT; A3GALT1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
Blood Group Antigen H Type 2; Monoclonal Antibody; Blood Group Antigen H Type 2 (CD173) Mouse Monoclonal Antibody; A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT; anti-ABO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
33C13
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
354
Applicable Applications for anti-ABO antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cell Surface
Immunogen
Mucin isolated from an ovarian cyst fluid
Hu-Chromosome Location
9q34.2
Positive Control
KG1 cells or human colorectal carcinoma.
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen H Type 2 Monoclonal Antibody (19-OLE))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen H Type 2 Monoclonal Antibody (19-OLE))
Related Product Information for anti-ABO antibody
This MAb preferably reacts with determinants of chain A and H type 3 (Gal 1-3GalNAc-R) and (Gal 1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This MAb is applicable for tissue staining in tumor patients with blood groups A and AP. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detect on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulationduring maligant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells and in secretion of certain indiviuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
References
Bara J, Daher N, Mollicone R, Oriol R. Immunohistological patterns of 20 monoclonal antibodies against non-A, non-B glycoconjugates in normal human pyloric and duodenal mucosae. Blood TransfImmunohae- matol. 1987; 30:685-692. Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 353-720, 1987. Norwalk Virus Binds to Histo-Blood Group Antigens Present on Gastroduodenal Epithelial Cells of Secretor Individuals. SEVERINE MARIONNEAU, NATHALIE RUVOE -N, BEATRICE LE MOULLAC-VAIDYE, MONIQUE CLEMENT, ANNE CAILLEAU-THOMAS, GUILLERMO RUIZ-PALACOIS, PENGWEI HUANG, XI JIANG, and JACQUES LE PENDU. GASTROENTEROLOGY 2002;122:1967-1977. Expression of Mucin Peptide and Blood Group ABH- and Lewis-Related Carbohydrate Antigens in Normal Human Conjunctiva. Catherine Garcher, Jacques Bara, Alain Bron, and Rafael Oriol. Invest Ophthalmol Vis Sci. 1994;35:1184-1191
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Multiple
NCBI Official Full Name
histo-blood group ABO system transferase
NCBI Official Synonym Full Names
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
NCBI Official Symbol
ABO
NCBI Official Synonym Symbols
GTB; NAGAT; A3GALNT; A3GALT1
NCBI Protein Information
histo-blood group ABO system transferase
UniProt Protein Name
Histo-blood group ABO system transferase
Protein Family
UniProt Gene Name
ABO
UniProt Synonym Gene Names
A transferase; B transferase
UniProt Entry Name
BGAT_HUMAN
NCBI Description
This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family.
Protein type: Transferase; Membrane protein, integral; EC 2.4.1.37; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.40
Chromosomal Location of Human Ortholog: 9q34.2
Disease: Blood Group, Abo System
Research Articles on ABO
Similar Products
Product Notes
The ABO abo (Catalog #AAA439652) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blood Group Antigen H Type 2 (CD173) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Blood Group Antigen H Type 2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Immunofluorescence (0.5-1ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ABO abo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blood Group Antigen H Type 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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