Typical Testing Data/Standard Curve (for reference only)
Human Adenylosuccinate synthetase isozyme 1 (ADSSL1) ELISA Kit | ADSSL1 elisa kit
Human Adenylosuccinate synthetase isozyme 1 (ADSSL1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADSS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADSS antibody and an ADSS-HRP conjugate. The assay sample and buffer are incubated together with ADSS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADSS concentration since ADSS from samples and ADSS-HRP conjugate compete for the anti-ADSS antibody binding site. Since the number of sites is limited, as more sites are occupied by ADSS from the sample, fewer sites are left to bind ADSS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADSS concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
ADSSL1 is a muscle isozyme of adenylosuccinate synthase (EC 6.3.4.4), which catalyzes the initial reaction in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) (Sun et al., 2005 [PubMed 15786719]).[supplied by OMIM, Dec 2008]
Uniprot Description
ADSSL1: Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP. Belongs to the adenylosuccinate synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; Nucleotide Metabolism - purine; EC 6.3.4.4
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: cytoplasm; cytosol
Molecular Function: actin filament binding; GTPase activity; protein homodimerization activity; GTP binding; magnesium ion binding; phosphate binding; adenylosuccinate synthase activity
Biological Process: AMP biosynthetic process; response to starvation; purine ribonucleoside monophosphate biosynthetic process; response to muscle activity; immune system process; nucleobase, nucleoside and nucleotide metabolic process; IMP metabolic process; glutamine metabolic process; aspartate metabolic process; purine base metabolic process
Research Articles on ADSSL1
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Product Notes
The Human ADSSL1 adssl1 (Catalog #AAA7204871) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7204871 ELISA Kit recognizes Human ADSSL1. It is sometimes possible for the material contained within the vial of "Adenylosuccinate synthetase isozyme 1 (ADSSL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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