Western Blot (WB)
(Left: Western Blot analysis of mouse thymocytes cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody. Right: Western Blot analysis of normal human peripheral blood cells cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody.)
Rat GILZ Monoclonal Antibody | anti-GILZ antibody
GILZ antibody
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Western Blot (WB)
(Left: Western Blot analysis of mouse thymocytes cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody. Right: Western Blot analysis of normal human peripheral blood cells cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody.)
Western Blot (WB)
(Left: Western Blot analysis of mouse thymocytes cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody. Right: Western Blot analysis of normal human peripheral blood cells cultured without (Lane 1) or with (Lane 2) dexamethasone for 3 hr. using GILZ antibody (10 ug/ml), followed by Rat IgG HRP antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GILZ: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities. Belongs to the TSC-22/Dip/Bun family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: nucleus; cytosol
Molecular Function: MRF binding; transcription factor activity
Biological Process: negative regulation of skeletal muscle development; regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; body fluid secretion; response to osmotic stress; transmembrane transport
Research Articles on GILZ
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Product Notes
The GILZ tsc22d3 (Catalog #AAA838335) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GILZ can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the GILZ tsc22d3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GILZ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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