Flow Cytometry
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Rat CCR7 Monoclonal Antibody | anti-CCR7 antibody
CCR7 antibody (biotin)
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Flow Cytometry
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using CCR7 antibodyStaining of C57BL/6 splenocytes with CD4 antibody (FITC) and 0.5 ug of Rat IgG2a kappa Isotype Control (biotin) (left) or 0.5 ug of CD197 antibody (biotin) (right) followed by Streptavidin (PE). Staining with CD197 antibody was carried out at 37deg C for 0.5 hours and Fc receptor binding was blocked by staining cells with CD16/CD32 antibody. Cells in the lymphocyte gate were used for analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Uniprot Description
CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q12-q21.2
Cellular Component: cell surface; integral to membrane; plasma membrane; intracellular; external side of plasma membrane
Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity
Biological Process: positive regulation of cell adhesion; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; positive regulation of JNK cascade; response to lipopolysaccharide; elevation of cytosolic calcium ion concentration; positive regulation of T cell receptor signaling pathway; positive regulation of humoral immune response; dendritic cell chemotaxis; establishment of T cell polarity; inflammatory response; positive regulation of cell-matrix adhesion; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of hypersensitivity; positive regulation of phosphoinositide 3-kinase activity; negative thymic T cell selection; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; positive regulation of pseudopodium formation; myeloid dendritic cell chemotaxis; positive regulation of actin filament polymerization; regulation of interferon-gamma production; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; ruffle organization and biogenesis; regulation of interleukin-1 beta secretion; immune response
Research Articles on CCR7
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Product Notes
The CCR7 ccr7 (Catalog #AAA833028) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CCR7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CCR7 ccr7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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