Rat Tyrosine-protein kinase JAK3 ELISA Kit | Jak3 elisa kit

Rat Tyrosine-protein kinase JAK3 ELISA Kit

Cat. Num. AAA762293

• Gene Names: JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Reactivity: Rat
• Synonyms: Tyrosine-protein kinase JAK3; Rat Tyrosine-protein kinase JAK3 ELISA Kit; Jak3/JAKL/LJAK/L-JAK/JAK-3/tyrosine-protein kinase JAK3/Janus kinase 3/Leukocyte janus kinase; Jak3 elisa kit

• Reactivity: Rat
• Sequence Length: 1124

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Jak3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Jak3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Jak3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Jak3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Jak3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47157315
• NCBI GeneID: 3718
• NCBI Accession #: NP_000206.2
• NCBI GenBank Nucleotide #: NM_000215.3
• Molecular Weight: 68,279 Da
• NCBI Official Full Name: tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: JAK3
• NCBI Official Synonym Symbols: JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• NCBI Protein Information: tyrosine-protein kinase JAK3
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: JAK3
• UniProt Synonym Gene Names: JAK-3; L-JAK
• UniProt Entry Name: JAK3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Uniprot Description

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; JakA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cytoskeleton; cytosol; endomembrane system; extrinsic to internal side of plasma membrane

Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding

Biological Process: activation of MAPKK activity; adaptive immune response; axon guidance; B cell differentiation; cell migration; elevation of cytosolic calcium ion concentration; enzyme linked receptor protein signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of dendritic cell cytokine production; negative regulation of FasL biosynthetic process; negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; negative regulation of T cell activation; negative regulation of T-helper 1 cell differentiation; nerve growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of activated T cell proliferation; positive regulation of calcium ion transport; positive regulation of immune response; positive regulation of T cell proliferation; protein amino acid phosphorylation; Ras protein signal transduction; small GTPase mediated signal transduction; STAT protein nuclear translocation; T cell homeostasis; tyrosine phosphorylation of STAT protein; tyrosine phosphorylation of Stat5 protein; vascular endothelial growth factor receptor signaling pathway

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative