Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody (FITC) and 0.5 ug of Rat IgG2a Isotype Control (biotin) (left) or 0.5 ug of BP-1 antibody (biotin) (right) followed by Streptavidin (PE). Samples were pre-incubated with CD16 antibody/32 (Purified)[ 14-0161 to block Fc receptors. Total viable cells were used for analysis.)
Rat BP1 Monoclonal Antibody | anti-BP1 antibody
BP1 antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
BP1; Monoclonal Antibody; BP1 antibody (biotin); Monoclonal BP1; Anti-BP1; BP-1; BP 1; Ly51; Ly-51; anti-BP1 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
6C3
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
240
Applicable Applications for anti-BP1 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody (FITC) and 0.5 ug of Rat IgG2a Isotype Control (biotin) (left) or 0.5 ug of BP-1 antibody (biotin) (right) followed by Streptavidin (PE). Samples were pre-incubated with CD16 antibody/32 (Purified)[ 14-0161 to block Fc receptors. Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody (FITC) and 0.5 ug of Rat IgG2a Isotype Control (biotin) (left) or 0.5 ug of BP-1 antibody (biotin) (right) followed by Streptavidin (PE). Samples were pre-incubated with CD16 antibody/32 (Purified)[ 14-0161 to block Fc receptors. Total viable cells were used for analysis.)
Related Product Information for anti-BP1 antibody
Rat monoclonal BP1 antibody (biotin)
Product Categories/Family for anti-BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
12,713 Da
NCBI Official Full Name
beta protein 1 BP1
UniProt Protein Name
Homeobox protein DLX-4
UniProt Gene Name
DLX4
UniProt Synonym Gene Names
BP1; DLX7; DLX8; DLX9
UniProt Entry Name
DLX4_HUMAN
Uniprot Description
DLX4: May play a role in determining the production of hemoglobin S. May act as a repressor. Belongs to the distal-less homeobox family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter
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Product Notes
The BP1 dlx4 (Catalog #AAA834475) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BP1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the BP1 dlx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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