Rabbit anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody

CD33 Rmab

Cat. Num. AAA370338

• Gene Names: CD33; p67; SIGLEC3; SIGLEC-3
• Reactivity: Human
• Synonyms: CD33; Monoclonal Antibody; CD33 Rmab; anti-CD33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: RBT-CD33
• Sequence Length: 183

• Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
• Immunogen: Recombinant protein encoding the extracellular domain of human CD33.
• Summary and Explanation: CD33 (gp62 or siglec-3) is a glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. The function of CD33 is not known, but it may have a role in cell-to-cell adhesion. In maturing granulocytic cells, there is progressive down-regulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation.; ; Detection of CD33 using monoclonal antibodies has been a critical component in immunophenotyping acute leukemias, particularly Acute Myeloid Leukemias.
• Localization: Membranous
• Control: Placenta, Myometrium, Lung, Colon, Spleen, Lymph Node, Tonsil, Acute Myeloid Leukemia
• Presentation: CD33 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
• Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.; 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 3. Unused solution should be disposed of according to local and federal regulations.; 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.; 5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (5).
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate (Please Inquire), ImmunoDNA Retriever with EDTA (Please Inquire) or ImmunoDNA Digestor (Please Inquire). See reverse side for complete protocol. Tissue should remain hydrated via use Immuno/DNA Washer solutions (Please Inquire). ; Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides (Please Inquire).; 2. Air dry for 2 hours at 58° C.; 3. Deparaffinize, dehydrate and rehydrate tissues.; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (Please Inquire) or EDTA (Please Inquire).; 5. Any of three heating methods may be used:; a. TintoRetriever Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with IHC wash buffer or DI water.; 9. Continue IHC staining protocol.
• Preparation and Storage: Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Immunohistochemistry

(Inset: IHC of CD33 on a FFPE Colon Tissue)

Immunohistochemistry Protocol

NCBI and Uniprot Product Information

• NCBI GI #: 1032656012
• NCBI GeneID: 945
• Molecular Weight: 25,293 Da
• NCBI Official Full Name: CD33 molecule transcript variant
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33
• UniProt Protein Name: Myeloid cell surface antigen CD33
• Protein Family: Myeloid cell surface antigen
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3; Siglec-3
• UniProt Entry Name: CD33_HUMAN

Uniprot Description

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell-cell signaling; negative regulation of cell proliferation; regulation of immune response; signal transduction