Human Hormone-Sensitive Lipase (HSL) ELISA Kit | HSL elisa kit

Human Hormone-Sensitive Lipase (HSL) ELISA Kit

Cat. Num. AAA3800751

• Gene Names: LIPE; HSL; LHS; AOMS4; FPLD6
• Reactivity: Human
• Synonyms: Hormone-Sensitive Lipase (HSL); Human Hormone-Sensitive Lipase (HSL) ELISA Kit; HSL elisa kit

• Reactivity: Human
• Sequence Length: 1076

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 100 U/L-1600 U/L
• Sensitivity: 10 U/L

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSL elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHSLin the sample, This HSLELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHSLconcentration. The concentration ofHSLin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21328446
• NCBI GeneID: 3991
• NCBI GenBank Nucleotide #: NP_005348.2
• UniProt Accession #: Q05469
• NCBI Official Full Name: hormone-sensitive lipase
• NCBI Official Synonym Full Names: lipase E, hormone sensitive type
• NCBI Official Symbol: LIPE
• NCBI Official Synonym Symbols: HSL; LHS; AOMS4; FPLD6
• NCBI Protein Information: hormone-sensitive lipase
• UniProt Protein Name: Hormone-sensitive lipase
• Protein Family: Hormone-sensitive lipase
• UniProt Gene Name: LIPE
• UniProt Synonym Gene Names: HSL
• UniProt Entry Name: LIPS_HUMAN

NCBI Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: EC 3.1.1.79; Lipase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4