FOXA2 Polyclonal Antibody | anti-FOXA2 antibody

Goat Polyclonal to Human FOXA2

Cat. Num. AAA242809

• Gene Names: FOXA2; HNF3B; TCF3B
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FOXA2; Polyclonal Antibody; Goat Polyclonal to Human FOXA2; Anti-FOXA2 Antibody (C-Terminus) IHC-plus; HNF3B; HNF-3-beta; HNF-3B; TCF3B; Transcription factor 3B; Forkhead box A2; Forkhead box protein A2; Hepatic nuclear factor-3-beta; TCF-3B; Human FOXA2; anti-FOXA2 antibody

• Host: FOXA2 antibody was produced in Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
• Clonality: Polyclonal
• Specificity: Human FOZA2. This antibody is expected to recognise both reported isoforms
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-FOXA2 antibody: Immunochemistry (IHC), Immunochemistry-Paraffin (IHC-P), Western Blot (WB), Peptide Enzyme-Linked Immunosorbet Assay
• Application Notes: IHC-P: 3.75ug/ml; WB: 1-3ug/ml; Peptide Enzyme-Linked Immunosorbent Assay: 1:4000; Optimal dilution to be determined by the researcher
• Target: FOXA2
• Family: Transcription factor/ not assigned-transcription factor
• Immunogen Species: FOXA2 antibody was rasied against Human
• Immunogen: FOXA2 antibody was raised against peptide with sequence C_GVYSRPIMNSS, from C-Terminus of protein sequence according to NP_068556.NP_710141.1.
• Epitope: aa453-463
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-FOXA2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of Human Liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Product Categories/Family for anti-FOXA2 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 194394143
• NCBI GeneID: 3170
• NCBI Accession #: NP_068556.2
• NCBI GenBank Nucleotide #: NM_021784.4
• UniProt Accession #: Q9Y261; Q8WUW4; Q96DF7
• NCBI Official Full Name: hepatocyte nuclear factor 3-beta isoform 1
• NCBI Official Synonym Full Names: forkhead box A2
• NCBI Official Symbol: FOXA2
• NCBI Official Synonym Symbols: HNF3B; TCF3B
• NCBI Protein Information: hepatocyte nuclear factor 3-beta; HNF-3B; TCF-3B; HNF-3-beta; forkhead box protein A2; transcription factor 3B; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta
• UniProt Protein Name: Hepatocyte nuclear factor 3-beta
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXA2
• UniProt Synonym Gene Names: HNF3B; TCF3B; HNF-3-beta; HNF-3B; TCF-3B
• UniProt Entry Name: FOXA2_HUMAN

NCBI Description

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

FOXA2: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. Binds DNA as a monomer. Binds TLE1. Interacts with FOXA1 and FOXA3. Interacts with PRKDC. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20p11

Cellular Component: cytoplasm; nucleus

Molecular Function: protein domain specific binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; adult locomotory behavior; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter by glucose; cell fate specification; negative regulation of glucokinase activity; cell differentiation in hindbrain; ectoderm formation; regulation of steroid metabolic process; positive regulation of smoothened signaling pathway; neuron fate specification; positive regulation of transcription from RNA polymerase II promoter by glucose; dorsoventral neural tube patterning; in utero embryonic development; negative regulation of transcription factor activity; chromatin modification; somite rostral/caudal axis specification; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; positive regulation of embryonic development; regulation of blood coagulation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell development