Mouse anti-Human Factor Va, light chain, fragment E, 74kD Monoclonal Antibody

Factor Va, light chain, fragment E, 74kD

Cat. Num. AAA604982

• Reactivity: Human
• Applications: ELISA, Western Blot, immunoassay, Radioimmunoassay
• Purity: Purified; Purified (>95% SDS-PAGE)
• Synonyms: Factor Va; light chain; fragment E; 74kD; Monoclonal Antibody; Anti -Factor Va; anti-Factor Va; 74kD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H368
• Specificity: Recognizes human Factor Va, light chain, (fragment E, 74,000). Does not react with intact Factor V.
• Purity/Purification: Purified; Purified (>95% SDS-PAGE)
• Form/Format: Supplied as a liquid, 50% glycerol, ddH2O.

• Applicable Applications for anti-Factor Va, light chain, fragment E, 74kD antibody: ELISA (EL/EIA), Western Blot (WB), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in ELISA, RIA and Western Blot.; Dilution: Western Blot: 5ug/ml
• Immunogen: Purified human Factor V
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Gel: Novex 4-12% Bis-Tris|Load: 1ug MBS604982 per lane|Buffer: MOPS|Standard: SeeBluePlus 2; Myosin (191kD), Phosphorylase B (97kD), BSA (64kD), Glutamic Dehydrogenase (51kD), Alcohol Dehydrogenase (39kD), Carbonic Anhydrase (28kD), Myoglobin Red (19kD), Lysozyme (14kD))

Related Product Information for anti-Factor Va, light chain, fragment E, 74kD antibody

Factor V is a large, single chain, plasma glycoprotein which is an essential component in the blood coagulation cascade (1). During coagulation, the procofactor, factor V, is converted to the active cofactor, factor Va, via limited proteolysis by the serine protease a-thrombin (illustrated above), and less efficiently by factor Xa. The active cofactor is composed of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain non-covalently associated in the presence of calcium ions. Factor Va serves as a cofactor for the serine protease factor Xa. Factor Va and Xa assemble on a phospholipid surface in a non-covalent and calcium ion-dependent manner, to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of the zymogen prothrombin to the active serine protease, a-thrombin. Assembly of the prothrombinase complex increases the rate at which prothrombin is converted to thrombin by nearly 300,000-fold relative to the rate with factor Xa alone. Down regulation of the prothrombinase complex is accomplished partly through the inactivation of factor Va by activated protein C.

Product Categories/Family for anti-Factor Va, light chain, fragment E, 74kD antibody

Antibodies; Abs to Coagulation Factors