GEL
(Gel: Novex 4-12% Bis-Tris|Load: T8678 (1ug/lane)|Buffer: MES|Standard: SeeBluePlus 2; Myosin (188kD), Phosphorylase B (98kD), BSA (62kD), Glutamic Dehydrogenase (49kD), Alcohol Dehydrogenase (38kD), Carbonic Anhydrase (28kD), Myoglobin Red (17kD), Lysozyme (14kD), Aprotinin (6kD), Insulin, B chain (3kD).)
Trypsin Inhibitor, Corn Active Protein | LOC541688 active protein
Trypsin Inhibitor, Corn (CTI)
Purity
Highly PurifiedCTI is purified using a combination of published procedures (3,4). CTI is extracted from the kernels of fresh sweet corn into a physiologic buffer. The extract is de-fatted using acetone. The protein is further purified by employing gel
Synonyms
Trypsin Inhibitor; Corn; Corn (CTI); LOC541688 active protein
Host
Corn kernels
Purity/Purification
Highly Purified
CTI is purified using a combination of published procedures (3,4). CTI is extracted from the kernels of fresh sweet corn into a physiologic buffer. The extract is de-fatted using acetone. The protein is further purified by employing gel
CTI is purified using a combination of published procedures (3,4). CTI is extracted from the kernels of fresh sweet corn into a physiologic buffer. The extract is de-fatted using acetone. The protein is further purified by employing gel
Form/Format
Supplied as a liquid in 20mM Tris, 0.3M sodium chloride, pH 7.4.
Specific Activity
~25 Units/mg
Extinction Coefficient
20.0
[E1%1cm, 280nm was determined by ultracentrifugation studies (3)].
[E1%1cm, 280nm was determined by ultracentrifugation studies (3)].
Unit Definition
Preparations of CTI are tested for the ability to prolong the aPTT assay without affecting the PT assay. One unit is defined as the amount of CTI required to double the aPTT of 1ml normal human plasma.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C/-80 degree C. Aliquots are stable for at least 6 months at -20 degree C/-80 degree C.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
GEL
(Gel: Novex 4-12% Bis-Tris|Load: T8678 (1ug/lane)|Buffer: MES|Standard: SeeBluePlus 2; Myosin (188kD), Phosphorylase B (98kD), BSA (62kD), Glutamic Dehydrogenase (49kD), Alcohol Dehydrogenase (38kD), Carbonic Anhydrase (28kD), Myoglobin Red (17kD), Lysozyme (14kD), Aprotinin (6kD), Insulin, B chain (3kD).)
GEL
(Gel: Novex 4-12% Bis-Tris|Load: T8678 (1ug/lane)|Buffer: MES|Standard: SeeBluePlus 2; Myosin (188kD), Phosphorylase B (98kD), BSA (62kD), Glutamic Dehydrogenase (49kD), Alcohol Dehydrogenase (38kD), Carbonic Anhydrase (28kD), Myoglobin Red (17kD), Lysozyme (14kD), Aprotinin (6kD), Insulin, B chain (3kD).)
Related Product Information for LOC541688 active protein
Corn Trypsin Inhibitor (CTI) is a small protein that is localized in the kernels of most species of corn. CTI is not only an inhibitor of trypsin, it is also a specific inhibitor of human Factor XIIa when tested in blood clotting experiments (1-6). The inhibitor forms a one-to-one complex with either trypsin or Factor XIIa. When added to plasma, it prolongs the activated partial thromboplastin time without affecting the PT assay (6). The specificity for Factor XIIa makes the inhibitor useful for the segregation and study of coagulation reactions (6).
The protein is a single chain molecule comprised of 112 amino acids (3,7). This yields a calculated molecular weight of 12,028D (7). The calculated molecular weight and that which has been determined by sedimentation equilibrium analyses of the purified protein (12,500D) are in good agreement (3). A comparison of the amino acid sequence of CTI to that of other trypsin or serine protease inhibitors reveals little or no similarities (7).
The protein is a single chain molecule comprised of 112 amino acids (3,7). This yields a calculated molecular weight of 12,028D (7). The calculated molecular weight and that which has been determined by sedimentation equilibrium analyses of the purified protein (12,500D) are in good agreement (3). A comparison of the amino acid sequence of CTI to that of other trypsin or serine protease inhibitors reveals little or no similarities (7).
Product Categories/Family for LOC541688 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,500
NCBI Official Full Name
trypsin inhibitor
NCBI Official Symbol
LOC541688
NCBI Protein Information
trypsin inhibitor
Similar Products
Product Notes
The LOC541688 (Catalog #AAA634314) is an Active Protein produced from Corn kernels and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Trypsin Inhibitor, Corn, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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