Cytochrome P450 24A1 (CYP24A1) Recombinant Protein | CYP24A1 recombinant protein

Recombinant Cytochrome P450 24A1 (CYP24A1)

Cat. Num. AAA2018602

• Gene Names: Cyp24a1; CP24; Cyp24; 24-OHase
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cytochrome P450 24A1 (CYP24A1); Recombinant Cytochrome P450 24A1 (CYP24A1); CYP24A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H KRLNTKVWQA HTLAWDTIFK SVKPCIDHRL ERYSQQPGAD FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ RLLREIQSVL PDNQTPRAED VRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYTLP KGTVLTLNTQ VLGSSEDNFE DADKFRPERW LEKEKKINPF AHLPFGVGKRMCIGRRLAEL QLHLALCWII QKYNIVATDS EPVEMLHLGI LVPSRELPIA FCPR
• Sequence Length: 514

• Applicable Applications for CYP24A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: His260~Arg514 (Accession # Q64441) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Mitochondrion
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753572
• NCBI GeneID: 13081
• NCBI Accession #: NP_034126.1
• NCBI GenBank Nucleotide #: NM_009996.3
• UniProt Accession #: Q64441
• Molecular Weight: 33.2kDa
• NCBI Official Full Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• NCBI Official Synonym Full Names: cytochrome P450, family 24, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp24a1
• NCBI Official Synonym Symbols: CP24; Cyp24; 24-OHase
• NCBI Protein Information: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450, 24; cytochrome P450 24A1; cytochrome P450-CC24; cytochrome P450, 24a1; vitamin D(3) 24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase
• UniProt Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Protein Family: 1,25-dihydroxyvitamin D(3) 24-hydroxylase
• UniProt Gene Name: Cyp24a1
• UniProt Synonym Gene Names: Cyp-24; Cyp24; 24-OHase
• UniProt Entry Name: CP24A_MOUSE

NCBI Description

The protein encoded by this gene localizes to the mitochondrion, where it degrades calcitriol to calcitetrol. This gene is upregulated by binding of calcitriol to the upstream regulatory region and to a downstream enhancer region, thereby allowing calcitriol to autoregulate its concentration in the cell. The encoded protein may also play a role in calcium homeostasis. [provided by RefSeq, Aug 2015]

Uniprot Description

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.126; Mitochondrial

Cellular Component: mitochondrion

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity; monooxygenase activity

Biological Process: response to vitamin D; vitamin D metabolic process