Vitronectin, Native Protein | VTN protein

Vitronectin, Human, Native

Cat. Num. AAA635237

• Gene Names: VTN; VN
• Purity: Purified; 95% by SDS-PAGE
• Synonyms: Vitronectin; Native; Human; VTN protein

• Host: Human plasma
• Purity/Purification: Purified; 95% by SDS-PAGE
• Form/Format: Supplied as a liquid in 50mM sodium phosphate, 150mM sodium chloride, pH 7.4.

• Extinction Coefficient: E1%1cm, 280nm=13.8
• Isoelectric Point: 4.75-5.25 (8M urea)
• Structure: Circulates in monomeric, dimeric and possibly higher oligomeric forms. Monomer: 459aa, single chain polypeptide with seven intrachain disulfides and one free sulfhydryl.
• Percent Carbohydrate: 10-15%
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

GEL

(Gel: Novex 4-12% Bis-Tris|Load: V2200A, 1ug per lane|Buffer: MOPS|Standard: SeeBluePlus 2; Myosin (191kD), Phosphorylase B (97kD), BSA (64kD), Glutamic Dehydrogenase (51kD), Alcohol Dehydrogenase (39kD), Carbonic Anhydrase (28kD), Myoglobin Red (19kD), Lysozyme (14kD))

Related Product Information for VTN protein

Vitronectin is a plasma glycoprotein that circulates in the blood at a concentration range of 200 to 400mg/ml constituting 0.2 to 0.5% of total plasma proteins (1,2). It is detected as a mixture of both 75kDa and 65kDa forms (3). The 65kDa form is a product of proteolytic processing of the former which appears to be endogenously present. Vitronectin is involved in a number of physiological processes that include blood coagulation, fibrinolysis, complement cascade, and cell adhesion. Vitronectin (Serum spreading factor) is one of the two major cell-adhesive glycoproteins.  It is a major ligand for the vitronectin receptor (aV/b3) or aV/b5 integrin on adhesive cells (4). Other functions include heparin-binding; collagen-binding; osteonectin-binding; complement lysis inhibitor and "scavenger" protein for macromolecular products of the complement and haemostasis cascade systems (5). It also functions as a substrate for trans glutaminase (Factor XIIIa) crosslinking, cAMP-dependent protein kinase, and tyrosyl protein sulfotransferase (4,5). Vitronectin has implications for thrombosis due to its heparin binding properties. It has been postulated that vitronectin prevents the acceleration of clot formation inhibition by acting as a heparin scavenger ultimately protecting thrombin and factor Xa from heparin-dependent inactivation by antithrombin III or heparin cofactor II (6,7). Vitronectin is also thought to play an important role in fibrinolysis by its ability to bind active PAI-1 (4). Extracellular binding of vitronectin to plasminogen activator inhibitor-1 (PAI-1) and plasminogen, stabilize the inhibitor and thus affect tissue plasminogen activator-mediated plasmin formation (4). 

The vitronectin domain structure starting at the NH4-terminus consists of a somatomedin-B region (residues 1 to 44), a connecting segment containing the major cell attachment RGD sequence (residues 45 to 47) and a highly acidic region (residues 53 to 64), followed by hemopexin-like repeats (homology to hemopexin which is a heme-binding plasma protein), a glycosaminoglycan-binding site which is represented by a 40-amino acid stretch rich in basic residues, a protease-sensitive region susceptible to high concentrations of thrombin, and a COOH-terminal protein kinase A-dependent phosphorylation site, which is adjacent to the protease cleavage site (4,5).
Vitronectin provides a molecular link important for cell adhesion and regulation of defense mechanisms in the terminal steps of blood coagulation and complement cascades

Product Categories/Family for VTN protein

Molecular Biology; MB-Vitronectin

NCBI and Uniprot Product Information

• NCBI GI #: 120564447
• NCBI GeneID: 397192
• UniProt Accession #: P48819
• Molecular Weight: 75,000
• NCBI Official Full Name: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN
• NCBI Protein Information: vitronectin; S-protein; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_PIG

Uniprot Description

Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway

By similarity.

Subunit structure: Monomer. Interacts with SERPINE1/PAI1 and C1QBP

By similarity.

Subcellular location: Secreted › extracellular space.

Tissue specificity: Plasma.

Domain: The SMB domain mediates interaction with SERPINE1/PAI1

By similarity.

Post-translational modification: Sulfated on 2 tyrosine residues

By similarity.N- and O-glycosylated

By similarity.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Sequence similarities: Contains 4 hemopexin repeats.Contains 1 SMB (somatomedin-B) domain.