GEL
(Gel: Novex 4-12% Bis-Tris; Load: M3896-90B1 Bovine Lactadherin, 1ug per lane; Buffer: MOPS|Standard: SeeBluePlus 2; Myosin (191kD), Phosphorylase B (97kD), BSA (64kD), Glutamic Dehydrogenase (51kD), Alcohol Dehydrogenase (39kD), Carbonic Anhydrase (28kD), Myoglobin Red (19kD), Lysozyme (14kD))
Milk Fat Globule-EGF Factor 8, Bovine Protein | mfge8a protein
Milk Fat Globule-EGF Factor 8, Bovine (MFG-E8, MFGM, Sperm Surface Protein SP47, MP47, Lactadherin) (FITC)
Gene Names
mfge8a; mfge8l; im:6895406; zgc:110846
Purity
Highly Purified~95% (SDS-PAGE)
Synonyms
Milk Fat Globule-EGF Factor 8; Bovine; Bovine (MFG-E8; MFGM; Sperm Surface Protein SP47; MP47; Lactadherin) (FITC); mfge8a protein
Host
Bovine Milk
Purity/Purification
Highly Purified
~95% (SDS-PAGE)
~95% (SDS-PAGE)
Form/Format
Supplied as a liquid TBS, pH 7.4, 1% BSA, 0.02% sodium azide. Labled with FITC.
Extinction Coefficient
E1%, 1cm, 280nm = 17.0
Structure
Single chain with two EGF domains and two C domains. The mouse isoform contains a central proline rich region and the human molecule contains a single EGF domain
Percent Carbohydrate
6%, 13% for glycosylation variants
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
GEL
(Gel: Novex 4-12% Bis-Tris; Load: M3896-90B1 Bovine Lactadherin, 1ug per lane; Buffer: MOPS|Standard: SeeBluePlus 2; Myosin (191kD), Phosphorylase B (97kD), BSA (64kD), Glutamic Dehydrogenase (51kD), Alcohol Dehydrogenase (39kD), Carbonic Anhydrase (28kD), Myoglobin Red (19kD), Lysozyme (14kD))
GEL
(Gel: Novex 4-12% Bis-Tris; Load: M3896-90B1 Bovine Lactadherin, 1ug per lane; Buffer: MOPS|Standard: SeeBluePlus 2; Myosin (191kD), Phosphorylase B (97kD), BSA (64kD), Glutamic Dehydrogenase (51kD), Alcohol Dehydrogenase (39kD), Carbonic Anhydrase (28kD), Myoglobin Red (19kD), Lysozyme (14kD))
Related Product Information for mfge8a protein
Lactadherin is a widely distributed glycoprotein (~ 50kD), which was originally characterized due to its association with milk fat/lipid globule membranes. Synonymous names are PAS-6/7, bovine-associated mucoprotein, BA-46, P47, and MFG-E8. Structural hallmarks of lactadherin are the presence of two epidermal growth factor (EGF) homology domains (with an RGD peptide motif in the second EGF domain), and two C domains sharing homology with the discoidin family of lectin domains including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin shows preferential binding to phosphatidylserine (L-form) in a calcium independent manner.
Purified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding.
Purified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding.
Product Categories/Family for mfge8a protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52000
NCBI Official Full Name
milk fat globule-EGF factor 8 protein a isoform 2
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein a
NCBI Official Symbol
mfge8a
NCBI Official Synonym Symbols
mfge8l; im:6895406; zgc:110846
NCBI Protein Information
milk fat globule-EGF factor 8 protein a; milk fat globule-EGF factor 8 protein, like
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Product Notes
The mfge8a (Catalog #AAA635091) is a Protein produced from Bovine Milk and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Milk Fat Globule-EGF Factor 8, Bovine, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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