Human Claudin 5 ELISA Kit | CLDN5 elisa kit

Human Claudin 5 ELISA Kit

Cat. Num. AAA765554

• Gene Names: CLDN5; AWAL; BEC1; TMVCF; CPETRL1
• Reactivity: Human
• Synonyms: Claudin 5; Human Claudin 5 ELISA Kit; CLDN5/AWAL/BEC1/CLDN5/CPETRL1/TMDVCF/TMVCF1/AWALTransmembrane protein deleted in VCFS/BEC1/claudin 5/claudin-5/CPETRL1/TMVCFTMDVCF/transmembrane protein deleted in velocardiofacial syndrome; CLDN5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLDN5. No significant cross-reactivity or interference between CLDN5 and analogues was observed.
• Sequence Length: 218

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 37589904
• NCBI GeneID: 7122
• Molecular Weight: 23,147 Da
• NCBI Official Full Name: Claudin 5
• NCBI Official Synonym Full Names: claudin 5
• NCBI Official Symbol: CLDN5
• NCBI Official Synonym Symbols: AWAL; BEC1; TMVCF; CPETRL1
• NCBI Protein Information: claudin-5
• UniProt Protein Name: Claudin-5
• Protein Family: Claudin
• UniProt Gene Name: CLDN5
• UniProt Synonym Gene Names: AWAL; TMVCF; TMDVCF
• UniProt Entry Name: CLD5_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: apicolateral plasma membrane; cortical actin cytoskeleton; integral to membrane; intercellular junction; tight junction

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion; intercellular junction assembly; learning; palate development; transforming growth factor beta receptor signaling pathway