Mouse Epidermal Growth Factor-like Protein 7 Monoclonal Antibody | anti-EGF-L7 antibody

Epidermal Growth Factor-like Protein 7 Monoclonal Antibody

Cat. Num. AAA190950

• Gene Names: EGFL7; NEU1; ZNEU1; VE-STATIN
• Applications: ELISA, Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: Epidermal Growth Factor-like Protein 7; Monoclonal Antibody; Epidermal Growth Factor-like Protein 7 Monoclonal Antibody; Anti-Epidermal Growth Factor-like Protein 7; anti-EGF-L7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody reacts with human EGFL-7 protein.
• Form/Format: 100ug, Protein A-purified, lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
• Sequence Length: 273

• Applicable Applications for anti-EGF-L7 antibody: ELISA (EIA), Immunoblot (IB), Immunohistochemistry (IHC)
• Application Notes: ELISA: Use at 1-10ug/ml.; Immunoblotting: Use at 1-5ug/ml. A band of ~30kDa is detected; Immunohistochemistry: Use at 2- 10ug/ml.
• Antigen: His-tagged EGFL-7 recombinant protein. Accession no. Q9UHF1.
• Preparation and Storage: This product is stable for at least one (1) year at -20 degree C to -70 degree C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Related Product Information for anti-EGF-L7 antibody

EGF-L7 (Epidermal Growth Factor Like Protein 7) is a multi-domain protein containing two EGF like domains and one EMI domain. It is expressed almost exclusively in endothelial cells and functions to promote normal development of the vascular system. EGF-L7 is capable of antagonistic binding to Notch receptors resulting in the inhibition of Notch signaling in HUVEC and neural stem cells. In research models inducing hypoxia and subsequent reoxygenation, (H/R), EGF-L7 can inhibit ICAM-1 expression and enhance the inhibition of NF-kappaB activation. Additionally, EGF-L7 is a chemoattractant for endothelial cells and binds to the extracellular matrix. The overexpression of EGF-L7 is observed in various cancers, and is generally correlated with increased metastasis and a poor prognosis.

Product Categories/Family for anti-EGF-L7 antibody

Growth Factors/Cytokines/Receptor Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 41393583
• NCBI GeneID: 51162
• NCBI Accession #: NP_958854.1
• NCBI GenBank Nucleotide #: NM_201446.2
• Molecular Weight: 29,618 Da
• NCBI Official Full Name: epidermal growth factor-like protein 7
• NCBI Official Synonym Full Names: EGF like domain multiple 7
• NCBI Official Symbol: EGFL7
• NCBI Official Synonym Symbols: NEU1; ZNEU1; VE-STATIN
• NCBI Protein Information: epidermal growth factor-like protein 7
• UniProt Protein Name: Epidermal growth factor-like protein 7
• Protein Family: Epidermal growth factor-like protein
• UniProt Gene Name: EGFL7
• UniProt Synonym Gene Names: MEGF7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
• UniProt Entry Name: EGFL7_HUMAN

NCBI Description

This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: calcium ion binding

Biological Process: angiogenesis; blood vessel development; cell adhesion; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; vasculogenesis