Mouse anti-Human ABCB4 Monoclonal Antibody | anti-ABCB4 antibody

ABCB4 Antibody

Cat. Num. AAA9230977

• Gene Names: ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: ABCB4; Monoclonal Antibody; ABCB4 Antibody; Multidrug resistance protein 3; 3.6.3.44; ATP-binding cassette sub-family B member 4; P-glycoprotein 3; MDR3; PGY3; anti-ABCB4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This ABCB4 antibody is generated from a mouse immunized with a recombinant protein of human ABCB4.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 1279

• Applicable Applications for anti-ABCB4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:4000
• Antigen Type: Recombinant Protein
• Antigen Source: Human
• Function: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:24045840). Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571, PubMed:28012258). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (By similarity).Cellular Location
• Cellular Location: Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Apical cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Membrane raft. Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000250|UniProtKB:Q08201}. Note=Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity) Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate-or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of canalicular plasma membranes (PubMed:23468132). {ECO:0000250|UniProtKB:P21440, ECO:0000269|PubMed:15258199, ECO:0000269|PubMed:19674157, ECO:0000269|PubMed:23468132}
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-ABCB4 Antibody at 1:4000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 142 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ABCB4 antibody

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

Product Categories/Family for anti-ABCB4 antibody

Crown Antibodies; Primary Antibodies; Metabolism; Signal Transduction

References

van der Bliek A.M.,et al.Gene 71:401-411 (1988). Hillier L.W.,et al.Nature 424:157-164 (2003). Scherer S.W.,et al.Science 300:767-772 (2003). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Smit J.J.,et al.Biochim. Biophys. Acta 1261:44-56 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4505771
• NCBI GeneID: 5244
• NCBI Accession #: NP_000434.1
• NCBI GenBank Nucleotide #: NM_000443.3
• UniProt Accession #: P21439; Q14813; A0A2V7; A4D1D3; A4D1D4; A4D1D5; D6W5P3; D6W5P4
• Molecular Weight: Calculated MW: 141523
• NCBI Official Full Name: phosphatidylcholine translocator ABCB4 isoform A
• NCBI Official Synonym Full Names: ATP binding cassette subfamily B member 4
• NCBI Official Symbol: ABCB4
• NCBI Official Synonym Symbols: GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• NCBI Protein Information: phosphatidylcholine translocator ABCB4
• UniProt Protein Name: Phosphatidylcholine translocator ABCB4
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB4

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.3.44; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: apical plasma membrane; cytoplasm; integral to plasma membrane; membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; phosphatidylcholine transmembrane transporter activity; phospholipid transporter activity; protein binding

Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; bile acid secretion; lipid homeostasis; lipid metabolic process; phospholipid translocation; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; positive regulation of cholesterol transport; response to drug; transmembrane transport; transport

Disease: Cholestasis, Intrahepatic, Of Pregnancy 3; Cholestasis, Progressive Familial Intrahepatic, 3; Gallbladder Disease 1