Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit | EGFL7 elisa kit

Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit

Cat. Num. AAA2022892

• Gene Names: EGFL7; NEU1; ZNEU1; VE-STATIN
• Reactivity: Mouse
• Synonyms: EGF Like Domain Protein; Multiple 7 (EGFL7); Mouse EGF Like Domain Protein; Multiple 7 (EGFL7) ELISA Kit; ZNEU1; MEGF7; Zneu1; Vascular endothelial statin; EGF Like Domain Containing 7; Epidermal growth factor-like protein 7; Multiple epidermal growth factor-like domains protein 7; EGFL7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGF Like Domain Protein, Multiple 7 (EGFL7).; No significant cross-reactivity or interference between EGF Like Domain Protein, Multiple 7 (EGFL7) and analogues was observed.
• Sequence Length: 273

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EGFL7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EGFL7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 41393583
• NCBI GeneID: 51162
• NCBI Accession #: NP_958854.1
• NCBI GenBank Nucleotide #: NM_201446.2
• UniProt Accession #: Q9UHF1; Q5M7Y5; Q5VUD5; Q96EG0; B3KRP0; M9VTX9
• Molecular Weight: 29,618 Da
• NCBI Official Full Name: epidermal growth factor-like protein 7
• NCBI Official Synonym Full Names: EGF-like-domain, multiple 7
• NCBI Official Symbol: EGFL7
• NCBI Official Synonym Symbols: NEU1; ZNEU1; VE-STATIN
• NCBI Protein Information: epidermal growth factor-like protein 7
• UniProt Protein Name: Epidermal growth factor-like protein 7
• Protein Family: Epidermal growth factor-like protein
• UniProt Gene Name: EGFL7
• UniProt Synonym Gene Names: MEGF7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
• UniProt Entry Name: EGFL7_HUMAN

NCBI Description

This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: calcium ion binding; Notch binding

Biological Process: blood vessel development; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; angiogenesis; vasculogenesis; cell adhesion; negative regulation of cell migration