<
Western Blot (WB)
(Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse skeletal muscle Lane 3: Mouse cerebellum Lane 4: Rat skin Lane 5: Daudi)
Rabbit EEF1A1 Monoclonal Antibody | anti-EEF1A1 antibody
EEF1A1 Antibody
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
EEF1A1; Monoclonal Antibody; EEF1A1 Antibody; CCS 3 antibody; CCS3 antibody; Cervical cancer suppressor 3 antibody; chunp6927 antibody; CTCL tumor antigen antibody; EE1A1 antibody; EEF 1 antibody; EEF1A antibody; eEF1A-1 antibody; EEF1A1 antibody; EF-1-alpha-1 antibody; EF-Tu antibody; EF1A antibody; EF1a like protein antibody; EF1A1_HUMAN antibody; Elongation factor 1 alpha subunit antibody; Elongation factor 1-alpha 1 antibody; Elongation factor Tu antibody; Eukaryotic elongation factor 1 A-1 antibody; Eukaryotic translation elongation factor 1 alpha 1 antibody; Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody; Glucocorticoid receptor AF 1 specific elongation factor antibody; GRAF 1EF antibody; HNGC:16303 antibody; ik:tdsubc_2a3 antibody; ik:tdsubc_2b3 antibody; LENG7 antibody; Leukocyte receptor cluster (LRC) member 7 antibody; Leukocyte receptor cluster member 7 antibody; Prostate tumor inducing protein 1 antibody; PTI1 antibody; tdsubc_2a3 antibody; Translation elongation factor 1 alpha 1 like 14 antibody; wu:fa91c07 antibody; wu:fa94b03 antibody; wu:fi13b09 antibody; xx:tdsubc_2a3 antibody; xx:tdsubc_2b3 antibody; anti-EEF1A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB44-13
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-EEF1A1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse skeletal muscle Lane 3: Mouse cerebellum Lane 4: Rat skin Lane 5: Daudi)
Western Blot (WB)
(Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse skeletal muscle Lane 3: Mouse cerebellum Lane 4: Rat skin Lane 5: Daudi)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-EEF1A1 antibody
The elongation factor-1 complex is composed of two subunits, EF-1 alpha1 (elongation factor 1-alpha 1) and EF-1 alpha2 (elongation factor 1-alpha 2), and is responsible for the delivery of aminoacyl tRNAs to the ribosome. EF-1 alpha1 is expressed predominately in brain, placenta, lung, liver, kidney and pancreas, while EF-1 alpha2 is highly expressed in heart, brain and skeletal muscle. Both EF-1 alpha1 and alpha2 localize to the nucleus and belong to the GTP-binding elongation factor family. The gene encoding EF-1 alpha2, which maps to human chromosome 20q13.3, may play a role in the development of ovarian cancer, while the EF-1 alpha1 gene, mapping to chromosome 6Q14.1, is commonly present as an autoantigen in patients with Felty syndrome. Felty syndrome is a disorder characterized by rheumatoid arthritis, a swollen spleen, decreased white blood cell count, and increased susceptibility to infection.
Product Categories/Family for anti-EEF1A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
462
NCBI Official Full Name
elongation factor 1-alpha 1
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol
EEF1A1
NCBI Official Synonym Symbols
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
NCBI Protein Information
elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte recep
UniProt Protein Name
Elongation factor 1-alpha 1
UniProt Gene Name
EEF1A1
UniProt Synonym Gene Names
EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
UniProt Entry Name
EF1A1_HUMAN
Similar Products
Product Notes
The EEF1A1 eef1a1 (Catalog #AAA9436595) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EEF1A1 eef1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
