Western Blot (WB)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human EEF1A1 Polyclonal Antibody | anti-EEF1A1 antibody
EEF1A1, CT (Elongation Factor 1-alpha 1, EF-1-alpha-1, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1 A-1, eEF1A-1, Elongation Factor Tu, EF-Tu, Leukocyte Receptor Cluster Member 7, LENG7, EF1A, EEF1A)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(EEF1A1 Antibody (C-term) (AP6592b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(EEF1A1 Antibody (C-term) (AP6592b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq]
Uniprot Description
EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protein type: Translation; Nucleolus; Translation elongation
Chromosomal Location of Human Ortholog: 6q14.1
Cellular Component: extracellular space; cortical actin cytoskeleton; membrane; eukaryotic translation elongation factor 1 complex; cytoplasm; nucleolus; nucleus; cytosol
Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity; tRNA binding
Biological Process: cellular protein metabolic process; translational elongation; transcription, DNA-dependent; translation; regulation of transcription, DNA-dependent; gene expression
Research Articles on EEF1A1
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Product Notes
The EEF1A1 eef1a1 (Catalog #AAA624911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1A1, CT (Elongation Factor 1-alpha 1, EF-1-alpha-1, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1 A-1, eEF1A-1, Elongation Factor Tu, EF-Tu, Leukocyte Receptor Cluster Member 7, LENG7, EF1A, EEF1A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the EEF1A1 eef1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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