Rabbit EEF1A1 Polyclonal Antibody | anti-EEF1A1 antibody

EEF1A1 Antibody

Cat. Num. AAA2524348

• Gene Names: EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: EEF1A1; Polyclonal Antibody; EEF1A1 Antibody; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; FLJ25721; GRAF-1EF; HNGC:16303; LENG7; MGC102687; MGC131894; MGC16224; PTI1; eEF1A-1; anti-EEF1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 462

• Applicable Applications for anti-EEF1A1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human EEF1A1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-28578
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EEF1A1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer using EEF1A1 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat testis using EEF1A1 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using EEF1A1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EEF1A1 antibody

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2, 3).

NCBI and Uniprot Product Information

• NCBI GI #: 4503471
• NCBI GeneID: 1915
• NCBI Accession #: NP_001393.1
• NCBI GenBank Nucleotide #: NM_001402.5
• Molecular Weight: 47,869 Da
• NCBI Official Full Name: elongation factor 1-alpha 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 alpha 1
• NCBI Official Symbol: EEF1A1
• NCBI Official Synonym Symbols: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• NCBI Protein Information: elongation factor 1-alpha 1; CTCL tumor antigen; EF-1-alpha-1; EF1a-like protein; cervical cancer suppressor 3; elongation factor 1 alpha subunit; elongation factor Tu; eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; leukocyte receptor cluster (LRC) member 7; leukocyte receptor cluster member 7; prostate tumor-inducing protein 1
• UniProt Protein Name: Elongation factor 1-alpha 1
• UniProt Gene Name: EEF1A1
• UniProt Synonym Gene Names: EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
• UniProt Entry Name: EF1A1_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Translation elongation; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: extracellular space; cortical actin cytoskeleton; eukaryotic translation elongation factor 1 complex; membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; tRNA binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; translation; gene expression