Mouse E-Cadherin Monoclonal Antibody | anti-Cdh1 antibody

E-Cadherin (C-terminal fragment)

Cat. Num. AAA474093

• Gene Names: Cdh1; Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: E-Cadherin; Monoclonal Antibody; E-Cadherin (C-terminal fragment); Uvomorulin; Cadherin-1; CTF1; CTF2; CTF3; CD324; Epithelial Cadherin; anti-Cdh1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Clone M533 was purified by Protein G affinity purification. The E-cadherin antibody detects a 120 kDa* protein corresponding to endogenous E-Cadherin expressed in human A431 cells.
• Purity/Purification: Protein G Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 884

• Applicable Applications for anti-Cdh1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:1000; WB: 1:250; WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
• Immunogen: Clone (M533) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the C-terminal region. This sequence is highly conserved in human and rat E-cadherin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-E-Cadherin (Cytoplasmic) at 1:1000 (lane 1) and 1:4000 (lane 2) and anti-E-Cadherin (C-terminal fragment) at 1:250 (lane 3) and 1:1000 (lane 4).)

Related Product Information for anti-Cdh1 antibody

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

References

Takeichi, M. (1988) Development 102:639.Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463.Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

NCBI and Uniprot Product Information

• NCBI GI #: 115419
• NCBI GeneID: 12550
• UniProt Accession #: P09803
• Molecular Weight: 120 kDa
• NCBI Official Full Name: Cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Official Synonym Symbols: Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_MOUSE

NCBI Description

This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Ubiquitin ligase

Cellular Component: apical junction complex; Golgi apparatus; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; catenin complex; trans-Golgi network; intercellular junction; actin cytoskeleton; cell-cell adherens junction; adherens junction; membrane; apical part of cell; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; metal ion binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; ankyrin binding; cell adhesion molecule binding; glycoprotein binding; protein phosphatase binding

Biological Process: in utero embryonic development; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of epithelial cell proliferation; trophectodermal cell differentiation; regulation of water loss via skin; cell-cell adhesion; sensory perception of sound; regulation of protein localization; synaptogenesis; protein metabolic process; calcium-dependent cell-cell adhesion; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; cell adhesion; homophilic cell adhesion; protein homooligomerization; negative regulation of epithelial cell proliferation