Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LETM1 domain-containing protein 1 (LETMD1) Standard Curve.)
Human LETM1 Domain-Containing Protein 1 (LETMD1) ELISA Kit | LETMD1 elisa kit
Human LETM1 Domain-Containing Protein 1 (LETMD1) ELISA Kit
Gene Names
LETMD1; HCCR; HCCR1; HCCR2; HCCR-1; HCCR-2; HCRR-2; SLC55A3; 1110019O13Rik
Reactivity
Human
Synonyms
LETM1 Domain-Containing Protein 1 (LETMD1); Human LETM1 Domain-Containing Protein 1 (LETMD1) ELISA Kit; LETMD1; 1110019O13Rik; DKFZp586A011; HCCR-2; HCCR1; cervical cancer 1 protooncogene; regulator of TP53; LETMD1 elisa kit
Reactivity
Human
Specificity
Human LETM1 domain-containing protein 1 (LETMD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LETMD1. No significant cross-reactivity or interference between Human LETMD1 and analogues was observed.
Sequence Length
360
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LETM1 domain-containing protein 1 (LETMD1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LETM1 domain-containing protein 1 (LETMD1) Standard Curve.)
Related Product Information for LETMD1 elisa kit
Description: This Human LETM1 domain-containing protein 1 (LETMD1) ELISA Kit employs a two-site sandwich ELISA to quantitate LETMD1 in samples. An antibody specific for LETMD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LETMD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LETMD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LETMD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms. HCCR-2 that was overexpressed in various human tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix. Ectopic expression of HCCR-2 resulted in direct tumorigenic conversions of NIH/3T3 and Rat1 fibroblasts. HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor.
Background: LETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms. HCCR-2 that was overexpressed in various human tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix. Ectopic expression of HCCR-2 resulted in direct tumorigenic conversions of NIH/3T3 and Rat1 fibroblasts. HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
LETM1 domain-containing protein 1
NCBI Official Synonym Full Names
LETM1 domain containing 1
NCBI Official Symbol
LETMD1
NCBI Official Synonym Symbols
HCCR; HCCR1; HCCR2; HCCR-1; HCCR-2; HCRR-2; SLC55A3; 1110019O13Rik
NCBI Protein Information
LETM1 domain-containing protein 1
UniProt Protein Name
LETM1 domain-containing protein 1
Protein Family
UniProt Gene Name
LETMD1
UniProt Entry Name
LTMD1_HUMAN
NCBI Description
This gene encodes a mitochondrial outer membrane protein. It has a potential role in tumorigenesis, which may result from negative regulation of the p53 tumor suppressor gene. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
LETMD1: Involved in tumorigenesis and may function as a negative regulator of the p53/TP53. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: mitochondrial outer membrane; integral to membrane
Molecular Function: protein binding
Research Articles on LETMD1
Similar Products
Product Notes
The Human LETMD1 letmd1 (Catalog #AAA9715491) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715491 ELISA Kit recognizes Human LETMD1. It is sometimes possible for the material contained within the vial of "LETM1 Domain-Containing Protein 1 (LETMD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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