E-Cadherin Recombinant Protein | CDH1 recombinant protein

E-Cadherin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122905

• Gene Names: Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649
• Purity: >85% as determined by SDS-PAGE
• Synonyms: E-Cadherin; E-Cadherin Protein; Mouse; Recombinant (His Tag); Mouse E-Cadherin/CDH1/E-cad/CD324 Protein (His Tag); AA960649 Protein; ARC-1 Protein; E-cad Protein; Ecad Protein; L-CAM Protein; Um Protein; UVO Protein; cadherin 1; type 1; E-cadherin (epithelial); CDH1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val709

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803)(Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH1 recombinant protein

Background: Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38kDa (E-CAD/CTF1), 33kDa (E-CAD/CTF2) and 29kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Product Categories/Family for CDH1 recombinant protein

Hemangioblast Markers Tumor Suppressors; Canonical Wnt Pathway

References

Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.

NCBI and Uniprot Product Information

• NCBI GI #: 115419
• NCBI GeneID: 12550
• UniProt Accession #: P09803; Q61377
• Molecular Weight: 884
• NCBI Official Full Name: Cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Official Synonym Symbols: Um; UVO; Ecad; E-cad; L-CAM; AA960649
• NCBI Protein Information: cadherin-1; ARC-1; E-cadherin; uvomorulin; epithelial cadherin
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_MOUSE

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Ubiquitin ligase

Cellular Component: apical junction complex; Golgi apparatus; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; catenin complex; trans-Golgi network; intercellular junction; actin cytoskeleton; cell-cell adherens junction; adherens junction; membrane; apical part of cell; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; metal ion binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; ankyrin binding; cell adhesion molecule binding; glycoprotein binding; protein phosphatase binding

Biological Process: in utero embryonic development; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of epithelial cell proliferation; trophectodermal cell differentiation; regulation of water loss via skin; cell-cell adhesion; sensory perception of sound; regulation of protein localization; synaptogenesis; protein metabolic process; calcium-dependent cell-cell adhesion; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; cell adhesion; homophilic cell adhesion; protein homooligomerization; negative regulation of epithelial cell proliferation