Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: LNCaP cell lysate
Lane 3.: A549 cell lysate )
Mouse anti-Human JAM2 Monoclonal Antibody | anti-JAM2 antibody
JAM2
Gene Names
JAM2; JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
JAM2; Monoclonal Antibody; Junctional adhesion molecule B; C21orf43; CD322; JAM-B; JAMB; PRO245; VE-JAM; VEJAM; anti-JAM2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT21D7
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
298
Applicable Applications for anti-JAM2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Immunogen
Recombinant human JAM2 (21-238aa) purified from E. coli.
Clone Note
Anti-human JAM2 mAb, clone AT21D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human JAM2 protein.
Preparation and Storage
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Lane 2.: LNCaP cell lysate Lane 3.: A549 cell lysate )
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: LNCaP cell lysate
Lane 3.: A549 cell lysate )
Related Product Information for anti-JAM2 antibody
JAM2, also known as CD322, belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. JAM2 is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs.
Product Categories/Family for anti-JAM2 antibody
References
Zhang Z., et al. (2004) Protein Sci. 13:2819-2824.
Liu T., et al. (2005) J. Proteome Res. 4:2070-2080.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,554 Da
NCBI Official Full Name
junctional adhesion molecule B isoform 1
NCBI Official Synonym Full Names
junctional adhesion molecule 2
NCBI Official Symbol
JAM2
NCBI Official Synonym Symbols
JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
NCBI Protein Information
junctional adhesion molecule B
UniProt Protein Name
Junctional adhesion molecule B
Protein Family
UniProt Gene Name
JAM2
UniProt Synonym Gene Names
C21orf43; VEJAM; JAM-B; JAM-2; VE-JAM
NCBI Description
This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Research Articles on JAM2
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Product Notes
The JAM2 jam2 (Catalog #AAA206622) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The JAM2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the JAM2 jam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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