CD358 recombinant protein

CD358 Recombinant Protein

Cat. Num. AAA3004347

• Gene Names: TNFRSF21; DR6; CD358; BM-018
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD358; CD358 Recombinant Protein; CD358 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: QPEQKASNLIGTYRHVDRATGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPVGTFTRHENGIEKCHDCSQPCPWPMIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGWGVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVIKPGTKETDNVCGTLPSFSSSTSPSPGTAIFPRPEHMETHEVPSSTYVPKGMNSTESNSSASVRPKVLSSIQEGTVPDNTSSARGKEDVNKTLPNLQVVNHQQGPHHRHILKLLPSMEATGGEKSSTPIKGPKRGHPRQNLHKHFDINEH

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 936
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for CD358 recombinant protein

Background: The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems. The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR6, also known as TNFRSF21, is a TNFR family member able to induce apoptosis as well as activation of NF-kappaB and JNK. Expression of DR6 is upregulated by NF-kappaB signaling. DR6 appears to play a critical role in the activation and differentiation of T and B lymphocytes. In the nervous system, beta-amyloid precursor protein (APP) activates DR6 to trigger neuronal degeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 21264087
• NCBI GeneID: 27242
• UniProt Accession #: O75509; Q0D2P5; Q96D86; B2RDI9
• Molecular Weight: 655
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 21
• NCBI Official Symbol: TNFRSF21
• NCBI Official Synonym Symbols: DR6; CD358; BM-018
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21; death receptor 6; TNFR-related death receptor 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• UniProt Gene Name: TNFRSF21
• UniProt Synonym Gene Names: DR6
• UniProt Entry Name: TNR21_HUMAN

NCBI Description

This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation. [provided by RefSeq, Jul 2013]

Uniprot Description

DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.

Protein type: Apoptosis; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: axon; integral to plasma membrane; plasma membrane; intrinsic to plasma membrane

Molecular Function: protein binding

Biological Process: myelination; neuron apoptosis; adaptive immune response; negative regulation of T cell proliferation; negative regulation of myelination; apoptosis; regulation of oligodendrocyte differentiation; B cell apoptosis; negative regulation of B cell proliferation; cellular lipid metabolic process; T cell receptor signaling pathway; humoral immune response