Rabbit Exo1 Polyclonal Antibody | anti-Exo1 antibody

Exo1 (K86) Polyclonal Antibody

Cat. Num. AAA3002746

• Gene Names: EXO1; HEX1; hExoI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Exo1; Polyclonal Antibody; Exo1 (K86) Polyclonal Antibody; Exonuclease 1; hExo1; Exonuclease I; hExoI; EXO1; EXOI; HEX1; anti-Exo1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Exo1 (K86) polyclonal antibody detects endogenous levels of Exo1 rotein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 803

• Applicable Applications for anti-Exo1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 55-05 of Human Exo1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Exo1 (K86) pAb at 1:500 dilutionLane1:The Testis tissue lysate of Rat (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:K562 whole cell lysate (10ug) Lane4:HEK293T whole cell lysate (10ug))

Related Product Information for anti-Exo1 antibody

Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the mExo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/ hEXO1 gene to chromosome 1q42-q43. mExo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The mismatch repair (MMR) system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MM. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 284055276
• NCBI GeneID: 9156
• NCBI Accession #: NP_003677.4
• NCBI GenBank Nucleotide #: NP_003677.4
• UniProt Accession #: Q9UQ84
• Molecular Weight: ~ 94kDa
• NCBI Official Full Name: exonuclease 1 isoform a
• NCBI Official Synonym Full Names: exonuclease 1
• NCBI Official Symbol: EXO1
• NCBI Official Synonym Symbols: HEX1; hExoI
• NCBI Protein Information: exonuclease 1
• UniProt Protein Name: Exonuclease 1
• Protein Family: Exonuclease
• UniProt Gene Name: EXO1
• UniProt Synonym Gene Names: EXOI; HEX1; hExo1; hExoI
• UniProt Entry Name: EXO1_HUMAN

NCBI Description

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: nucleoplasm; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; structure-specific DNA binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; DNA binding; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity; 5'-3' exodeoxyribonuclease activity; metal ion binding; exonuclease activity; ribonuclease H activity; flap endonuclease activity

Biological Process: humoral immune response mediated by circulating immunoglobulin; mismatch repair; meiotic cell cycle; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination