Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human TNFRSF10B(CD262) expression on Jurkat cells. Cells were stained with purified anti-Human TNFRSF10B(CD262), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human DR5/TRAIL R2 Monoclonal Antibody | anti-TNFRSF10B antibody
Recombinant Anti-DR5/TRAIL R2 Antibody, Rabbit Monoclonal
Gene Names
TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
DR5/TRAIL R2; Monoclonal Antibody; Recombinant Anti-DR5/TRAIL R2 Antibody; Rabbit Monoclonal; TRAIL R2/CD262/TNFRSF10B Antibody; Rabbit MAb; tumor necrosis factor receptor superfamily; member 10b; Anti-CD262 Antibody; Anti-DR5 Antibody; Anti-KILLER Antibody; Anti-KILLER/DR5 Antibody; Anti-TRAIL-R2 Antibody; Anti-TRAILR2 Antibody; Anti-TRICK2 Antibody; Anti-TRICK2A Antibody; Anti-TRICK2B Antibody; Anti-TRICKB Antibody; Anti-ZTNFR9 Antibody; anti-TNFRSF10B antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
102
Specificity
Human TNFRSF10B/TRAILR2/CD262/DR5.
No cross-reactivity in ELISA with Human TNFRSF10A/CD261/APO2, Human TNFRSF10D/CD264/DCR2/TRAILR4
No cross-reactivity in ELISA with Human TNFRSF10A/CD261/APO2, Human TNFRSF10D/CD264/DCR2/TRAILR4
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNFRSF10B antibody
ELISA (EIA), Flow Cytometry (FC/FACS/FCM)
Application Notes
ELISA: 1:5000-1:10,000
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD262 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNFRSF10B/CD262 extracellular domain (rh TNFRSF10B).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human TNFRSF10B(CD262) expression on Jurkat cells. Cells were stained with purified anti-Human TNFRSF10B(CD262), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human TNFRSF10B(CD262) expression on Jurkat cells. Cells were stained with purified anti-Human TNFRSF10B(CD262), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-TNFRSF10B antibody
Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
Product Categories/Family for anti-TNFRSF10B antibody
References
Schneider P, et al. (1997) TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB. Immunity. 7(6): 831-6.
Ichikawa K, et al. (2003) TRAIL-R2 (DR5) mediates apoptosis of synovial fibroblasts in rheumatoid arthritis. J Immunol. 171(2): 1061-9.
Walczak H, et al. (1997) TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J. 16(17): 5386-97.
Ichikawa K, et al. (2003) TRAIL-R2 (DR5) mediates apoptosis of synovial fibroblasts in rheumatoid arthritis. J Immunol. 171(2): 1061-9.
Walczak H, et al. (1997) TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J. 16(17): 5386-97.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,083 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily, member 10b isoform 1
NCBI Official Synonym Full Names
TNF receptor superfamily member 10b
NCBI Official Symbol
TNFRSF10B
NCBI Official Synonym Symbols
DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 10B
Protein Family
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
Research Articles on TNFRSF10B
Similar Products
Product Notes
The TNFRSF10B (Catalog #AAA8102897) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-DR5/TRAIL R2 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DR5/TRAIL R2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS/FCM). ELISA: 1:5000-1:10,000 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the TNFRSF10B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DR5/TRAIL R2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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