TERF1 / TRF1 recombinant protein

Recombinant Human TERF1 / TRF1 Protein (His tag)

Cat. Num. AAA2545426

• Gene Names: TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: TERF1 / TRF1; Recombinant Human TERF1 / TRF1 Protein (His tag); FLJ41416; PIN2; TRBF1; TRF; TRF1; hTRF1-AS; t-TRF1; TERF1 / TRF1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Sequence Length: 419

• Application Notes: The recombinant human TERF1 consists of 438 amino acids and predicts a molecular mass of 50.5 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TERF1 / TRF1 recombinant protein

Background: Telomeric repeat binding factor 1 (TRF1), also known as TERF1, the shelterin complex, which modulates the telomere structures. TRF1 protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Pin2/TRF1 was originally identified as a protein bound to telomeric DNA (TRF1) and as a protein involved in mitotic regulation (Pin2). Pin2/TRF1 negatively regulates telomere length and importantly, its function is tightly regulated during the cell cycle, acting as an important regulator of mitosis. TRF1 can be bound and modulated by two nucleolar GTP-binding proteins, nucleostemin (NS) and guanine nucleotide binding protein-like 3-like (GNL3L), which exhibit apparently opposite effects on the protein degradation of TRF1. TRF1/TERF1 may has association with cancer. TRF1 may play a significant role in cell differentiation in non-small cell lung cancer (NSCLC). The expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer. TRF1 expression in malignant gliomas cells, may play a role in the malignant progression of astroglial brain tumors.

Description: A DNA sequence encoding the human TERF1 isoform 2 (NP_003209.2) (Met 1-Asp 419) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 189409140
• NCBI GeneID: 7013
• NCBI Accession #: NP_003209.2
• NCBI GenBank Nucleotide #: NM_003218.3
• UniProt Accession #: P54274; Q15553; Q8NHT6; Q93029; A7XP29
• Molecular Weight: 48,239 Da
• NCBI Official Full Name: telomeric repeat-binding factor 1 isoform 2
• NCBI Official Synonym Full Names: telomeric repeat binding factor 1
• NCBI Official Symbol: TERF1
• NCBI Official Synonym Symbols: TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• NCBI Protein Information: telomeric repeat-binding factor 1
• UniProt Protein Name: Telomeric repeat-binding factor 1
• UniProt Gene Name: TERF1
• UniProt Synonym Gene Names: PIN2; TRBF1; TRF; TRF1

NCBI Description

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA bending activity; double-stranded telomeric DNA binding; protein binding; protein homodimerization activity; telomerase activity; telomeric DNA binding; ubiquitin binding

Biological Process: negative regulation of DNA replication; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere capping; telomere maintenance; telomere maintenance via telomerase; telomeric loop formation