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Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using NRF2 mouse mAb (1:2000 diluted).Predicted band size:68KDa.Observed band size:68KDa.)

Mouse NRF2 Monoclonal Antibody | anti-NRF2 antibody

Anti-NRF2 Mouse mAb

Gene Names
NFE2L2; NRF2; HEBP1
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
NRF2; Monoclonal Antibody; Anti-NRF2 Mouse mAb; erythroid derived 2 antibody; HEBP1 antibody; like 2 antibody; NF E2 related factor 2 antibody; NF-E2-related factor 2 antibody; NF2L2_HUMAN antibody; NFE2 related factor 2 antibody; NFE2-related factor 2 antibody; Nfe2l2 antibody; Nrf 2 antibody; NRF2 antibody; Nuclear factor (erythroid derived 2) like 2 antibody; Nuclear factor antibody; nuclear factor erythroid 2 like 2 antibody; Nuclear factor erythroid 2 related factor 2 antibody; Nuclear factor erythroid 2-related factor 2 antibody; Nuclear factor erythroid derived 2 like 2 antibody; anti-NRF2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
5F10-G4-B3
Purity/Purification
Affinity Purified
Form/Format
Purified antibody in PBS with o.o5% sodium azide.
Applicable Applications for anti-NRF2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500-1/2000
IHC: 1/200-1/1000
ELISA: 1/10000
Antibody
Primary antibody
Immunogen
Purified recombinant fragment of human NFE2L2 expressed in E. Coli.
Conjugation
Unconjugated
Modification
Unmodification
Cross Reactivity
Human
Subcellular Location
Cytoplasm, Nucleus
Preparation and Storage
Store at 4°C short term. Store at -20°C. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using NRF2 mouse mAb (1:2000 diluted).Predicted band size:68KDa.Observed band size:68KDa.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using NRF2 mouse mAb (1:2000 diluted).Predicted band size:68KDa.Observed band size:68KDa.)

Western Blot (WB)

(Western blot analysis using NFE2L2 mAb against HEK293 (1) and NFE2L2 (AA)

Western Blot (WB) (Western blot analysis using NFE2L2 mAb against HEK293 (1) and NFE2L2 (AA)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using NFE2L2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using NFE2L2 mouse mAb with DAB staining.)

Western Blot (WB)

(Western Blot analysis using NFE2L2 nAb against human NFE2L2 (AA:356-589) recombinant protein. (Expected MW is 52.1kDa).)

Western Blot (WB) (Western Blot analysis using NFE2L2 nAb against human NFE2L2 (AA:356-589) recombinant protein. (Expected MW is 52.1kDa).)

Western Blot (WB)

(Western blot analysis of extracts from Hela and MCF cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa. Observed band size: 68kDa.)

Western Blot (WB) (Western blot analysis of extracts from Hela and MCF cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa. Observed band size: 68kDa.)
Related Product Information for anti-NRF2 antibody
Entrez Summary: This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67.8kDa
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor, erythroid 2 like 2
NCBI Official Symbol
NFE2L2
NCBI Official Synonym Symbols
NRF2; HEBP1
NCBI Protein Information
nuclear factor erythroid 2-related factor 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2; NFE2-related factor 2

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: centrosome; cytoplasm; cytosol; nucleus; plasma membrane

Molecular Function: DNA binding; protein binding; protein domain specific binding; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; transcription from RNA polymerase II promoter; unfolded protein response

Research Articles on NRF2

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Product Notes

The NRF2 nfe2l2 (Catalog #AAA476699) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NRF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500-1/2000 IHC: 1/200-1/1000 ELISA: 1/10000. Researchers should empirically determine the suitability of the NRF2 nfe2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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