DR5/TRAIL R2 Recombinant Protein | TNFRSF10B recombinant protein

DR5/TRAIL R2 Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8118635

• Gene Names: TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: DR5/TRAIL R2; DR5/TRAIL R2 Protein; Human; Recombinant (His & hFc Tag); Human TRAIL R2/CD262/TNFRSF10B Protein (His & Fc Tag); tumor necrosis factor receptor superfamily; member 10b; CD262 Protein; DR5 Protein; KILLER Protein; KILLER/DR5 Protein; TRAIL-R2 Protein; TRAILR2 Protein; TRICK2 Protein; TRICK2A Protein; TRICK2B Protein; TRICKB Protein; ZTNFR9 Protein; TNFRSF10B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 375aa
• Sequence: Met1-Glu182

• Species: Human
• Tag: His & hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile56
• Protein Construction: |A DNA sequence encoding the human TNFRSF10B (NP_003833.3) extracellular domain (Met 1-Glu 182) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFSF10 at 10ug/ml (100ul/well) can bind human TNFRSF10B Fc chimera with a linear range of 2.5-40ng/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for TNFRSF10B recombinant protein

Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.

Product Categories/Family for TNFRSF10B recombinant protein

Cancer Drug Targets; Death Receptor Signaling

References

Schneider P, et al. (1997) TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB. Immunity. 7(6): 831-6.
Ichikawa K, et al. (2003) TRAIL-R2 (DR5) mediates apoptosis of synovial fibroblasts in rheumatoid arthritis. J Immunol. 171(2): 1061-9.
Walczak H, et al. (1997) TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J. 16(17): 5386-97.

NCBI and Uniprot Product Information

• NCBI GI #: 22547116
• NCBI GeneID: 8795
• NCBI Accession #: NP_003833.3
• NCBI GenBank Nucleotide #: NM_003842.3
• Molecular Weight: 45,083 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily, member 10b isoform 1
• NCBI Official Synonym Full Names: TNF receptor superfamily member 10b
• NCBI Official Symbol: TNFRSF10B
• NCBI Official Synonym Symbols: DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10B
• Protein Family: Tumor necrosis factor receptor superfamily

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]