Rabbit DNAJC15 Monoclonal Antibody | anti-DNAJC15 antibody

DNAJC15 Rabbit Monoclonal Antibody

Cat. Num. AAA3014127

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A affinity purified
• Synonyms: DNAJC15; Monoclonal Antibody; DNAJC15 Rabbit Monoclonal Antibody; DnaJ homolog subfamily C member 15; Cell growth-inhibiting gene 22 protein; Methylation-controlled J protein; MCJ; DNAJD1; GIG22; HSD18; anti-DNAJC15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of DNAJC15 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 150

• Applicable Applications for anti-DNAJC15 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DNAJC15 Rabbit mAb at 1:1000 dilutionLane4:The liver tissue lysate of Human)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DNAJC15 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DNAJC15 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-DNAJC15 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DNAJC15 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-DNAJC15 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-DNAJC15 antibody

The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of a J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. MCJ (methylation-controlled J protein), also known as HSD18, DNAJD1 or DNAJC15, is a 150 amino acid ubiquitously expressed single-pass membrane protein containing one J domain.

NCBI and Uniprot Product Information

• NCBI GI #: 66472920
• NCBI Accession #: NP_037370.2
• NCBI GenBank Nucleotide #: NP_037370.2
• UniProt Accession #: Q9Y5T4
• Molecular Weight: ~ 16kDa
• NCBI Official Full Name: dnaJ homolog subfamily C member 15
• UniProt Protein Name: DnaJ homolog subfamily C member 15
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJC15
• UniProt Synonym Gene Names: DNAJD1; MCJ
• UniProt Entry Name: DJC15_HUMAN

Uniprot Description

DNAJC15: Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP. Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9. Interacts with the TIM23 complex. Directly interacts with PAM16/MAGMAS; this interaction counteracts DNAJC15-dependent stimulation of HSPA9 ATPase activity. Associates with complex I of the mitochondrial electron transfer chain; this interaction may interfere with the formation of supercomplexes that facilitate the transfer of electrons between complexes

Protein type: Mitochondrial; Chaperone; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: protein transport; regulation of lipid metabolic process; cellular response to starvation; negative regulation of protein complex assembly