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SDS-Page

DNAJC15 recombinant protein

DNAJC15, 58-150aa, Human, His tag, E Coli

Gene Names
DNAJC15; MCJ; HSD18; DNAJD1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
DNAJC15; 58-150aa; Human; His tag; E Coli; DnaJ homolog subfamily C member 15; DNAJD1; HSD18; MCJ; DNAJC15 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSFRIWKPL EQVITETAKK ISTPSFSSYY KGGFEQKMSR REAGLILGVS PSAGKAKIRT AHRRVMILNH PDKGGSPYVA AKINEAKDLL ETTTKH
Sequence Length
150
Applicable Applications for DNAJC15 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for DNAJC15 recombinant protein
DNAJC15 contains 1 J domain. This protein is expressed ubiquitously and located on the membrane. DNAJC15 is absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin. Recombinant human DNAJC15 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DNAJC15 recombinant protein
References
Noskova L, Stranecky V, et al. (2011). Am J Hum Genet. 89 (2):241-52.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12.8 kDa (116aa) confirmed by MALDI-TOF
NCBI Official Full Name
dnaJ homolog subfamily C member 15
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member C15
NCBI Official Symbol
DNAJC15
NCBI Official Synonym Symbols
MCJ; HSD18; DNAJD1
NCBI Protein Information
dnaJ homolog subfamily C member 15
UniProt Protein Name
DnaJ homolog subfamily C member 15
Protein Family
UniProt Gene Name
DNAJC15
UniProt Synonym Gene Names
DNAJD1; MCJ

Uniprot Description

Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.

Research Articles on DNAJC15

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Product Notes

The DNAJC15 dnajc15 (Catalog #AAA204887) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DNAJC15 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DNAJC15 dnajc15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSFRIWKPL EQVITETAKK ISTPSFSSYY KGGFEQKMSR REAGLILGVS PSAGKAKIRT AHRRVMILNH PDKGGSPYVA AKINEAKDLL ETTTKH. It is sometimes possible for the material contained within the vial of "DNAJC15, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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