Rabbit anti-Human ACER1 Polyclonal Antibody | anti-ACER1 antibody

ACER1, CT (ACER1, ASAH3, Alkaline ceramidase 1, Acylsphingosine deacylase 3, N-acylsphingosine amidohydrolase 3) (PE)

Cat. Num. AAA6270924

• Gene Names: ACER1; ASAH3; ALKCDase1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACER1; Polyclonal Antibody; CT (ACER1; ASAH3; Alkaline ceramidase 1; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3) (PE); N-acylsphingosine amidohydrolase 3; anti-ACER1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ACER1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ACER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the C-terminal region of human ACER1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACER1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ACER1 antibody detected the ACER1 protein (arrow).)

Related Product Information for anti-ACER1 antibody

Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).

Product Categories/Family for anti-ACER1 antibody

Antibodies; Enzymes

References

Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008). Toulza, E., et al. Genome Biol. 8 (6), R107 (2007): Houben, E., et al. J. Lipid Res. 47(5):1063-1070(2006). Mao, C., et al. J. Biol. Chem. 278(33):31184-31191(2003). Ito, M., et al. Tanpakushitsu Kakusan Koso 47 (4 SUPPL), 455-462 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 19424128
• NCBI GeneID: 125981
• NCBI Accession #: NP_597999.1
• NCBI GenBank Nucleotide #: NM_133492.2
• UniProt Accession #: Q8TDN7; Q8R4X1
• Molecular Weight: 31,095 Da
• NCBI Official Full Name: alkaline ceramidase 1
• NCBI Official Synonym Full Names: alkaline ceramidase 1
• NCBI Official Symbol: ACER1
• NCBI Official Synonym Symbols: ASAH3; ALKCDase1
• NCBI Protein Information: alkaline ceramidase 1; alkCDase 1; alkaline CDase 1; acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3
• UniProt Protein Name: Alkaline ceramidase 1
• Protein Family: Alkaline ceramidase
• UniProt Gene Name: ACER1
• UniProt Synonym Gene Names: ASAH3; AlkCDase 1; Alkaline CDase 1
• UniProt Entry Name: ACER1_HUMAN

NCBI Description

Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010]

Uniprot Description

ACER1: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids. Belongs to the alkaline ceramidase family.

Protein type: Membrane protein, integral; Hydrolase; Lipid Metabolism - sphingolipid; Endoplasmic reticulum; Membrane protein, multi-pass; EC 3.5.1.23

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: ceramidase activity

Biological Process: keratinocyte differentiation; epidermis development; regulation of lipid metabolic process; sphingolipid metabolic process; response to alkalinity; sphingolipid biosynthetic process; sphingosine biosynthetic process; ceramide catabolic process; cell differentiation