NF-kappaB Antibody Kit

NF-kappaB Pathway Sampler Kit

Cat. Num. AAA2550051

• Applications: Western Blot
• Synonyms: NF-kappaB; NF-kappaB Pathway Sampler Kit; NF-kappaB antibody kit

• Applicable Applications for NF-kappaB antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of 293T cells using IKKalpha Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of KB cells with Phospho-IKKalpha (Thr23) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of COS-7 cells with Phospho-IkappaB-alpha (Ser32/S36) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of 293T cells using IkappaB-alpha Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of NIH-3T3 cells with Phospho-IKKalpha/beta (Ser176/177) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with Phospho-IKKalpha/beta (Ser180/181) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with IKKbeta Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of KB, Hela, 293T, 3T3 cells using NFkappaB-p65 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of COLO205 cells with Phospho-NFkappaB-p65 (Ser536) Polyclonal Antibody at dilution of 1:2000)

Related Product Information for NF-kappaB antibody kit

Background: The transcriptional nuclear factor kappaB (NF-kappaB)/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Activation occurs via phosphorylation of IkappaBalpha at Ser32 and Ser36, resulting in the ubiquitin-mediated proteasome- dependent degradation of IkappaBalpha and the release and nuclear translocation of active NF-kappaB dimers. The regulation of IkappaBbeta and IkappaBepsilon is similar to that of IkappaBalpha, however, the phosphorylation and degradation of these proteins occurs with much slower kinetics. Phosphorylation of IkappaBbeta occurs at Ser/Thr19 and Ser23, while IkappaBepsilon can be phosphorylated at Ser18 and Ser22. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex, consisting of three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunits of the kinase. Activation of IKK depends on phosphorylation at Ser177 and Ser181 in the activation loop of IKKbeta (176 and 180 in IKKalpha). NF-kappaB-inducing kinase (NIK), TANK-binding kinase 1 (TBK1), and its homolog IKKepsilon (IKKi), phosphorylate and activate IKKalpha and IKKbeta. The NF-kappaB family of transcription factors is comprised of five proteins in mammals, p65/RelA, c-Rel, RelB, NF-kappaB1 (p105/p50) and NF-kappaB2 (p100/p52). p105 and p100 are proteolytically processed to produce p50 and p52, respectively. The 50 kDa active form is produced through proteolytic processing following IKK-mediated phosphorylation of p105 at multiple sites (Ser922, 924, 928 and 933), while p100's processing to p52 is induced by phosphorylation of Ser864 and Ser868. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. Phosphorylation of p65/RelA at Ser276 by PKA C and MSK1 enhances transcriptional activity. p65 phosphorylation at Ser536 regulates activation, nuclear localization, protein-protein interactions, and transcriptional activity. PMA-induced NF-kappaB transcriptional activity is dependent on the region of p65 containing the potential phosphorylation sites Ser457, Thr458, Thr464 and Ser468. Phosphorylation of Ser468 by GSK-3beta inhibits basal p65 activity.