Immunocytochemistry (ICC)
(Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594)B - cell nuclei stained with DAPI (blue)C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue))
Mouse DDDDK Tag Monoclonal Antibody
Anti-DDDDK Tag Purified
Reactivity
Tagged fusion proteins in all species
Applications
Immunocytochemistry, Western Blot
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
DDDDK Tag; Monoclonal Antibody; Anti-DDDDK Tag Purified; anti-DDDDK Tag antibody
Host
Mouse
Reactivity
Tagged fusion proteins in all species
Clonality
Monoclonal
Isotype
IgG1
Clone Number
F-tag-01
Specificity
The mouse monoclonal antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-DDDDK Tag antibody
Immunocytochemistry (ICC), Western Blot (WB)
Immunogen
Synthetic peptide: DYKDDDDK conjugated to KLH
Preparation and Storage
Store at 2-8 degree C.
Immunocytochemistry (ICC)
(Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594)B - cell nuclei stained with DAPI (blue)C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue))
Immunocytochemistry (ICC)
(Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594)B - cell nuclei stained with DAPI (blue)C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue))
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Product Notes
The DDDDK Tag (Catalog #AAA1800624) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-DDDDK Tag Purified reacts with Tagged fusion proteins in all species and may cross-react with other species as described in the data sheet. AAA Biotech's DDDDK Tag can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the DDDDK Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDDDK Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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