Testing Data
(Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.)
Mouse anti-Human CCR4 Monoclonal Antibody | anti-CCR4 antibody
anti-human CCR4 monoclonal antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using KH- 4F5 Cat.# GM-0602. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.)
Testing Data #2
(Fig.2: Spectral Confocal Microscopy of CHO cells using KH-4F5 Cat.# GM-0602. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Testing Data #2
(Fig.2: Spectral Confocal Microscopy of CHO cells using KH-4F5 Cat.# GM-0602. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Testing Data #3
(SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Testing Data #3
(SDS-PAGE analysis of KH-4F5 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Murdoch C and Finn A (2000). Chemokine receptors and their role in inflammation and infectious diseases. Blood 95 (10): 3032-3043
Imai T et al. (1997). The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem 6;272(23):15036-42
Imai T et al. (1998). Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem 16;273(3):1764-8
Purandare AV and Somerville JE (2006). Antagonists of CCR4 as immunomodulatory agents. Curr Top Med Chem 6(13):1335-44
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]
Uniprot Description
CCR4: High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol- calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3p24
Cellular Component: cell soma; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: C-C chemokine receptor activity; chemokine receptor activity
Biological Process: response to antibiotic; G-protein coupled receptor protein signaling pathway; response to radiation; elevation of cytosolic calcium ion concentration; positive regulation of positive chemotaxis; response to bacterium; neuron migration; immune response; inflammatory response; chemotaxis; tolerance induction
Research Articles on CCR4
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Product Notes
The CCR4 ccr4 (Catalog #AAA468021) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CCR4 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCR4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA. Immunofluorescence: 1 ug/10^6 cells Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CCR4 ccr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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