Typical Testing Data/Standard Curve (for reference only)
Mouse Bone Morphogenetic Protein 9 ELISA Kit | BMP9 elisa kit
Mouse Bone Morphogenetic Protein 9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BMP9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP9 antibody and an BMP9-HRP conjugate. The assay sample and buffer are incubated together with BMP9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP9 concentration since BMP9 from samples and BMP9-HRP conjugate compete for the anti-BMP9 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP9 from the sample, fewer sites are left to bind BMP9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
GDF2: Could be involved in bone formation. Belongs to the TGF-beta family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q11.22
Cellular Component: extracellular space
Molecular Function: protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; BMP signaling pathway; positive regulation of interleukin-8 production; regulation of apoptosis; positive regulation of endothelial cell differentiation; angiogenesis; vasculogenesis; positive regulation of BMP signaling pathway; ossification; negative regulation of blood vessel endothelial cell migration; patterning of blood vessels; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of angiogenesis; positive regulation of angiogenesis; negative regulation of DNA replication; cartilage development; regulation of MAPKKK cascade; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell development; growth
Disease: Telangiectasia, Hereditary Hemorrhagic, Type 5
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Product Notes
The Mouse BMP9 gdf2 (Catalog #AAA742405) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742405 ELISA Kit recognizes Mouse BMP9. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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