Rabbit anti-Human ATP6V0B Polyclonal Antibody | anti-ATP6V0B antibody

ATP6V0B, ID (ATP6V0B, ATP6F, V-type proton ATPase 21kD proteolipid subunit, Vacuolar proton pump 21kD proteolipid subunit, hATPL) (Azide free) (HRP)

Cat. Num. AAA6276638

• Gene Names: ATP6V0B; ATP6F; HATPL; VMA16
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ATP6V0B; Polyclonal Antibody; ID (ATP6V0B; ATP6F; V-type proton ATPase 21kD proteolipid subunit; Vacuolar proton pump 21kD proteolipid subunit; hATPL) (Azide free) (HRP); hATPL; anti-ATP6V0B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP6V0B
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ATP6V0B antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 104-131 in the N-terminal region of human ATP6V0B. Species sequence homology: bovine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6000951. This demonstrates that MBS6000951 detected the EKI2 protein (arrow).)

Immunohistochemistry (IHC)

(iimmunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using MBS6000951 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000951 for immunohistochemistry.)

Product Categories/Family for anti-ATP6V0B antibody

Antibodies; Enzymes; ATPase

References

1. Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007). 2. Rojas, J.D., et al. Biochem. Biophys. Res. Commun. 320(4):1123-1132(2004). 3. Morel, N. Biol. Cell 95(7):453-457(2003). 4. Smith, A.N., et al. Mol. Cell 12(4):801-803(2003). 5. Izumi, H., et al. Biochim. Biophys. Acta 1628(2):97-104(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 86792634
• NCBI GeneID: 533
• NCBI Accession #: NP_001034546.1; NP_004038.1
• NCBI GenBank Nucleotide #: NM_001039457.2
• UniProt Accession #: Q99437; Q6IB32; D3DPY5
• Molecular Weight: 16,206 Da
• NCBI Official Full Name: V-type proton ATPase 21 kDa proteolipid subunit isoform 2
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
• NCBI Official Symbol: ATP6V0B
• NCBI Official Synonym Symbols: ATP6F; HATPL; VMA16
• NCBI Protein Information: V-type proton ATPase 21 kDa proteolipid subunit
• UniProt Protein Name: V-type proton ATPase 21 kDa proteolipid subunit
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0B
• UniProt Synonym Gene Names: ATP6F; V-ATPase 21 kDa proteolipid subunit
• UniProt Entry Name: VATO_HUMAN

NCBI Description

This gene encodes a portion of the V0 domain of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Activity of this enzyme is necessary for such varied processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

ATP6V0B: Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase proteolipid subunit family.

Protein type: Membrane protein, integral; Transporter, ion channel; Transporter; Transporter, iron; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: phagocytic vesicle membrane; vacuolar membrane; integral to membrane; endosome membrane

Molecular Function: transporter activity; hydrogen ion transmembrane transporter activity

Biological Process: interaction with host; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport