Typical Testing Data/Standard Curve (for reference only)
Human TGF-alpha ELISA Kit | TGF-alpha elisa kit
Human TGF-alpha (Transforming Growth Factor alpha) ELISA Kit
Reactivity
Human
Synonyms
TGF-alpha; Human TGF-alpha (Transforming Growth Factor alpha) ELISA Kit; TGF-alpha elisa kit
Reactivity
Human
Specificity
This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.
Sequence Length
173
Samples
Serum, Plasma, Urine. Please Consult Technical Support For The Applicability If Other Biological Fluids Need To Be Tested
Assay Type
Quantitative Sandwich
Detection Range
0.14-100ng/Ml
Sensitivity
0.13ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TF were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TF were tested on 3 different plates,20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TGF-alpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TF concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Samples (or Standards) and biotinylated detection antibody specific for Human TF are added to the micro ELISA plate wells. Human TF would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Transferrin is an iron binding protein found in blood plasma that is critical to cell health. Transferrin supplies iron to cells naturally in the body and as a supplement in cell culture media Transferrin (Tf) carries ferric iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body [1]. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. Transferrin has a molecular weight of around 80 kDa and contains two specific high-affinity Fe (III) binding sites. The affinity of transferrin for Fe (III) is extremely high (association constant is 1020M-1 at pH 7.4) but decreases progressively with decreasing pH below neutrality [2]. When not bound to iron, transferrin is known as Apo transferrin.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Samples (or Standards) and biotinylated detection antibody specific for Human TF are added to the micro ELISA plate wells. Human TF would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Transferrin is an iron binding protein found in blood plasma that is critical to cell health. Transferrin supplies iron to cells naturally in the body and as a supplement in cell culture media Transferrin (Tf) carries ferric iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body [1]. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. Transferrin has a molecular weight of around 80 kDa and contains two specific high-affinity Fe (III) binding sites. The affinity of transferrin for Fe (III) is extremely high (association constant is 1020M-1 at pH 7.4) but decreases progressively with decreasing pH below neutrality [2]. When not bound to iron, transferrin is known as Apo transferrin.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,279 Da
NCBI Official Full Name
TGF-alpha, partial
UniProt Protein Name
TGF-alpha
UniProt Gene Name
tgfa
UniProt Entry Name
D7RNZ2_GRYBI
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Product Notes
The Human TGF-alpha tgfa (Catalog #AAA2505418) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505418 ELISA Kit recognizes Human TGF-alpha. It is sometimes possible for the material contained within the vial of "TGF-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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