Rabbit RNASEH2C Polyclonal Antibody | anti-RNASEH2C antibody

RNASEH2C Rabbit Polyclonal

Cat. Num. AAA7603563

• Gene Names: RNASEH2C; AGS3; AYP1
• Reactivity: Human; other species are not tested.
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: RNASEH2C; Polyclonal Antibody; RNASEH2C Rabbit Polyclonal; AGS3; AYP1; Ribonuc ease H2 subunit C; ribonuclease H2; subunit C; Ribonuclease HI subunit C; RNase H1 small subunit; RNase H2 subunit C; anti-RNASEH2C antibody

• Host: Rabbit
• Reactivity: Human; other species are not tested.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Please decide the specificity by homology
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 164

• Applicable Applications for anti-RNASEH2C antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:200-1:1000; IHC: 1:20-1:200; IP: 1:500-1:1000
• Samples: Urine, Serum, Plasma, Tissue Extracts, Cell Lysate, Cell Culture Media And Other Biological Fluids Samples
• Detection Range: 2 umol/L-200 umol/L
• Preparation and Storage: Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain using MBS7603563 (RNASEH2C antibody) at dilution of 1:50)

Western Blot (WB)

(human testis tissue were subjected to SDS PAGE followed by western blot with MBS7603563 (RNASEH2C antibody) at dilution of 1:600)

Related Product Information for anti-RNASEH2C antibody

Background/Introduction: Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3-as a measure for NO level. The reaction products can be measured at a colorimetric readout at 550 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 38176285
• NCBI GeneID: 84153
• NCBI Accession #: NP_115569.2
• NCBI GenBank Nucleotide #: NM_032193.3
• NCBI Official Full Name: ribonuclease H2 subunit C
• NCBI Official Synonym Full Names: ribonuclease H2 subunit C
• NCBI Official Symbol: RNASEH2C
• NCBI Official Synonym Symbols: AGS3; AYP1
• NCBI Protein Information: ribonuclease H2 subunit C
• UniProt Protein Name: Ribonuclease H2 subunit C
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASEH2C
• UniProt Synonym Gene Names: AYP1; RNase H2 subunit C; AGS3
• UniProt Entry Name: RNH2C_HUMAN

NCBI Description

This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RNASEH2C: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2C are the cause of Aicardi-Goutieres syndrome type 3 (AGS3). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase H2 subunit C family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleus

Molecular Function: ribonuclease H activity

Biological Process: RNA catabolic process

Disease: Aicardi-goutieres Syndrome 3