Western Blot (WB)
(Western Blot Analysis:|Representative lot data.|O-GlcNAc transferease transfected HEK293 cell lysate (lane 1) and O-GlcNAcase transfected transfected HEK293 cell lysate (lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS605885 (1:1000 dilution of this lot). |Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Mouse anti-Human N-acetylglucosamine Monoclonal Antibody
N-acetylglucosamine (O-linked; O-GlcNAc) transferase [OGT, FLJ23071, HRNT1, MGC22921, O-GLCNAC, -O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase, O-linke
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
N-acetylglucosamine; Monoclonal Antibody; N-acetylglucosamine (O-linked; O-GlcNAc) transferase [OGT; FLJ23071; HRNT1; MGC22921; O-GLCNAC; -O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; O-linke; Anti -N-acetylglucosamine (O-linked; O-link; anti-N-acetylglucosamine antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10F624
Specificity
Recognizes human O-GlcNAc.0.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM NaCl, 0.05% sodium azide.
Applicable Applications for anti-N-acetylglucosamine antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
SANM-conjugated linear peptide corresponding to human O-GlcNAc.
Positive Control
HEK293 cell lysate.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis:|Representative lot data.|O-GlcNAc transferease transfected HEK293 cell lysate (lane 1) and O-GlcNAcase transfected transfected HEK293 cell lysate (lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS605885 (1:1000 dilution of this lot). |Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Western Blot (WB)
(Western Blot Analysis:|Representative lot data.|O-GlcNAc transferease transfected HEK293 cell lysate (lane 1) and O-GlcNAcase transfected transfected HEK293 cell lysate (lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS605885 (1:1000 dilution of this lot). |Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Related Product Information for anti-N-acetylglucosamine antibody
O-linked N-acetylglucosamine (O-GlcNAc), is a member of the O-GlcNAc transferase family. O-GlcNAc is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine/threonine hydroxyl groups. It plays an important role in nutrient sensing, gene expression, and protein degradation. O-GlcNAc is widely expressed in the pancreas and is present in smaller amounts in skeletal muscle, heart, brain and placenta.
Product Categories/Family for anti-N-acetylglucosamine antibody
Similar Products
Product Notes
The N-acetylglucosamine (Catalog #AAA605885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The N-acetylglucosamine (O-linked; O-GlcNAc) transferase [OGT, FLJ23071, HRNT1, MGC22921, O-GLCNAC, -O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase, O-linke reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's N-acetylglucosamine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the N-acetylglucosamine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-acetylglucosamine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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