Mouse anti-Human Cytokeratin 8 & 18 Monoclonal Antibody | anti-KRT8 antibody

Cytokeratin 8 & 18

Cat. Num. AAA370056

• Gene Names: KRT8; K8; KO; CK8; CYK8; K2C8; CARD2
• Reactivity: Human
• Synonyms: Cytokeratin 8 & 18; Monoclonal Antibody; anti-KRT8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: B22.1&B23.1
• Form/Format: Cytokeratin 8 & 18 is a cocktail of mouse monoclonal antibodies derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Control: Prostate, Pancreas, Salivary Gland
• Localization: Cytoplasmic
• Reactivity Note: Paraffin, Frozen
• Preparation and Storage: Store at 2 to 8 degree C in the dark.

Dilution Information

Immunohistochemistry (IHC)

Related Product Information for anti-KRT8 antibody

Cytokeratin 8 belongs to the Type II (basic) subfamily of high molecular-weight keratins and exists in combination with Cytokeratin 18 (Type I [acidic] subfamily of low molecular weight keratins). They are perhaps the most commonly found products of the intermediate filament gene family, and are expressed in single-layer epithelial tissues of the body. Cytokeratins 8 and 18 can be found in most simple epithelium (e.g., thyroid, female breast, gastrointestinal tract, and respiratory tract). Adenocarcinomas and most Non-keratinizing Squamous Carcinomas will stain, but Keratinizing Squamous Carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells will stain. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.

NCBI and Uniprot Product Information

• NCBI GI #: 181573
• NCBI GeneID: 3856
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; Q7L4M3; Q969I0
• Molecular Weight: 53,704 Da
• NCBI Official Full Name: cytokeratin 8
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8; CK-8; keratin-8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Uniprot Description

Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Ref.19

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Ref.13 Ref.14 Ref.17 Ref.19 Ref.20 Ref.22 Ref.30

Subcellular location: Cytoplasm. Nucleus › nucleoplasm

By similarity. Nucleus matrix

By similarity Ref.13 Ref.30.

Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Ref.13 Ref.19

Post-translational modification: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. Ref.12

Involvement in disease: Defects in KRT8 are a cause of cirrhosis (CIRRH) [

MIM:215600]. Ref.34

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.