Mouse CytokeRatin 4 / KeRatin K4 Monoclonal Antibody | anti-KRT4 antibody
Mouse anti CytokeRatin 4 / KeRatin K4
Gene Names
KRT4; K4; CK4; CYK4; FLJ31692; KRT4
Reactivity
Canine, Feline, Human, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
CytokeRatin 4 / KeRatin K4; Monoclonal Antibody; Mouse anti CytokeRatin 4 / KeRatin K4; anti-KRT4 antibody
Host
Mouse
Reactivity
Canine, Feline, Human, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6B10
Specificity
6B10 reacts exlcusively with cytokeRatin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stRatified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-KRT4 antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
6B10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytokeRatin prepaRation extracted from Human esophagus.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-KRT4 antibody
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-KRT4 antibody
References
van Muijen, G. N., Ruiter, D. J., Franke, W. W., Achtstatter, T., Haasnoot, W. H., Ponec, M., and Warnaar, S. O. (1986). Cell type heterogeneity of cytokeRatin expression in complex epithelia and carcinomas as demonstRated by monoclonal antibodies specific for cytokeRatins nos. 4 and 13, Exp Cell Res 162, 97-113. Weikel, W., Wagner, R., and Moll, R. (1987). Characterization of subcolumnar reserve cells and other epithelia of Human uterine cervix. DemonstRation of diverse cytokeRatin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeRatins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with keRatin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). CytokeRatins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102. Corver, W. E., Koopman, L. A., van der Aa, J., Regensburg, M., Fleuren, G. J., and Cornelisse, C. J. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intRatumor heterogeneity in cervical cancer, Cytometry 39, 96-107.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,285 Da
NCBI Official Full Name
cytokeratin 4 (408 AA)
NCBI Official Synonym Full Names
keratin 4
NCBI Official Symbol
KRT4
NCBI Official Synonym Symbols
K4; CK4; CYK4; FLJ31692; KRT4
NCBI Protein Information
keratin, type II cytoskeletal 4; CK-4; cytokeratin 4; cytokeratin-4; type-II keratin Kb4
UniProt Protein Name
Keratin, type II cytoskeletal 4
Protein Family
UniProt Gene Name
KRT4
UniProt Synonym Gene Names
CYK4
UniProt Entry Name
K2C4_HUMAN
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]
Uniprot Description
K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: intermediate filament cytoskeleton; cell surface; keratin filament; intermediate filament; nucleus
Molecular Function: protein binding; structural molecule activity
Biological Process: epithelial cell differentiation; cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation
Disease: White Sponge Nevus 1
Research Articles on KRT4
1. Infection by HPV stimulates the expression of cytokeratin 4
2. Data found that Plk1 binds and directly phosphorylates the HsCYK-4 at the midzone and this modification creates a major docking site for the tandem BRCT repeats of the Rho GTP exchange factor Ect2.
3. Failure to phosphorylate HsCyk-4 blocks Ect2 recruitment to the central spindle and the subsequent induction of furrowing.
4. 2 new mutations were found in the keratin 4 gene, the heterozygous missense mutation 1829G-->A in exon 2B, and 2324A-->G in non-coding region, which may be related with the development of WSN.
2. Data found that Plk1 binds and directly phosphorylates the HsCYK-4 at the midzone and this modification creates a major docking site for the tandem BRCT repeats of the Rho GTP exchange factor Ect2.
3. Failure to phosphorylate HsCyk-4 blocks Ect2 recruitment to the central spindle and the subsequent induction of furrowing.
4. 2 new mutations were found in the keratin 4 gene, the heterozygous missense mutation 1829G-->A in exon 2B, and 2324A-->G in non-coding region, which may be related with the development of WSN.
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Product Notes
The KRT4 krt4 (Catalog #AAA570035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 4 / KeRatin K4 reacts with Canine, Feline, Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CytokeRatin 4 / KeRatin K4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). 6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the KRT4 krt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CytokeRatin 4 / KeRatin K4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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