Mouse CYP2C9 Monoclonal Antibody | anti-CYP2C9 antibody

CYP2C9 antibody

Cat. Num. AAA533116

• Gene Names: CYP2C9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: CYP2C9 antibody was purified by affinity chromatography.
• Synonyms: CYP2C9; Monoclonal Antibody; CYP2C9 antibody; Monoclonal CYP2C9; Anti-CYP2C9; Cytochrome P450 Family 2 Subfamily C Polypeptide 9; CPC9; CYP2C; CYP2C10; MGC149605; MGC88320; P450 MP-4; P450 PB-1; P450IIC9; Cytochrome P450; Cytochrome P 450; Cytochrome P-450; anti-CYP2C9 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3D2
• Purity/Purification: CYP2C9 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 490

• Applicable Applications for anti-CYP2C9 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:2000
• Biological Significance: CYP2C9 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that CYP2C9 gene is polymorphic.
• Cross-Reactivity: Human
• Immunogen: CYP2C9 antibody was raised in mouse using a full length recombinant protein of human CYP2C9 (NP_000762) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CYP2C9 protein (Right) or empty vector (Left) detected with CYP2C9 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CYP2C9 protein in paraffin embedded Carcinoma of Human liver tissue using CYP2C9 antibody)

Related Product Information for anti-CYP2C9 antibody

Mouse monoclonal CYP2C9 antibody

Product Categories/Family for anti-CYP2C9 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 13699818
• NCBI GeneID: 1559
• NCBI Accession #: NP_000762
• NCBI GenBank Nucleotide #: NM_000771.3
• UniProt Accession #: P11712; P11713; Q16756; Q16872; Q5VX92; Q6IRV8; Q8WW80
• Molecular Weight: 18,005 Da
• NCBI Official Full Name: cytochrome P450 2C9
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily C, polypeptide 9
• NCBI Official Symbol: CYP2C9
• NCBI Official Synonym Symbols: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• NCBI Protein Information: cytochrome P450 2C9
• UniProt Protein Name: Cytochrome P450 2C9
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C9
• UniProt Synonym Gene Names: CYP2C10
• UniProt Entry Name: CP2C9_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2C9: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Lipid Metabolism - arachidonic acid; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Secondary Metabolites Metabolism - limonene and pinene degradation; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: (S)-limonene 6-monooxygenase activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; (S)-limonene 7-monooxygenase activity; arachidonic acid epoxygenase activity; oxidoreductase activity; steroid hydroxylase activity; drug binding; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; amide metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; drug metabolic process; monocarboxylic acid metabolic process; urea metabolic process

Disease: Coumarin Resistance