Rabbit FHIT Polyclonal Antibody | anti-FHIT antibody

Anti-FHIT Antibody

Cat. Num. AAA7606938

• Gene Names: FHIT; FRA3B; AP3Aase
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: FHIT; Polyclonal Antibody; Anti-FHIT Antibody; AP3Aase; FRA3B; anti-FHIT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid PBS with 0.02% sodium azide and 50% glycerol pH 7.3
• Sequence Length: 147

• Applicable Applications for anti-FHIT antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: FHIT
• Preparation and Storage: Store at -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart tissue slide using MBS7606938 (FHIT antibody) at dilution of 1:200)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7606938 at dilution of 1:1000)

Related Product Information for anti-FHIT antibody

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 261278358
• NCBI GeneID: 2272
• NCBI Accession #: NP_001159715.1
• NCBI GenBank Nucleotide #: NM_001166243.2
• UniProt Accession #: P49789; Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9
• Molecular Weight: 35kDa
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase isoform 1
• NCBI Official Synonym Full Names: fragile histidine triad
• NCBI Official Symbol: FHIT
• NCBI Official Synonym Symbols: FRA3B; AP3Aase
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: FHIT
• UniProt Synonym Gene Names: AP3Aase

NCBI Description

The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]

Uniprot Description

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: DNA replication; EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Tumor suppressor

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: cytoplasm; cytosol; extracellular exosome; fibrillar center; mitochondrion; plasma membrane

Molecular Function: bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; nucleotide binding; protein binding; ubiquitin protein ligase binding

Biological Process: intrinsic apoptotic signaling pathway by p53 class mediator; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process; regulation of transcription, DNA-templated; transcription, DNA-dependent