Human Protocadherin Beta 16 ELISA Kit | PCDHbeta16 elisa kit

Human Protocadherin Beta 16 ELISA Kit

Cat. Num. AAA760247

• Gene Names: PCDHB16; ME1; PCDH3X; PCDHB8a; PCDH-BETA16
• Reactivity: Human
• Synonyms: Protocadherin Beta 16; Human Protocadherin Beta 16 ELISA Kit; PCDHb16 ; PCDHbeta16 elisa kit

• Reactivity: Human
• Sequence Length: 776

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PCDHbeta16 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PCDHbeta16 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PCDHbeta16 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PCDHbeta16 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PCDHbeta16 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 605052038
• NCBI GeneID: 57717
• NCBI Accession #: NP_066008.2
• NCBI GenBank Nucleotide #: NM_020957.3
• Molecular Weight: 84,983 Da
• NCBI Official Full Name: protocadherin beta-16
• NCBI Official Synonym Full Names: protocadherin beta 16
• NCBI Official Symbol: PCDHB16
• NCBI Official Synonym Symbols: ME1; PCDH3X; PCDHB8a; PCDH-BETA16
• NCBI Protein Information: protocadherin beta-16
• UniProt Protein Name: Protocadherin beta-16
• UniProt Gene Name: PCDHB16
• UniProt Synonym Gene Names: KIAA1621; PCDH3X; PCDH-beta-16
• UniProt Entry Name: PCDBG_HUMAN

NCBI Description

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHB16: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: calcium-dependent cell-cell adhesion; homophilic cell adhesion; synaptic transmission; synaptogenesis