Immunocytochemistry (ICC)/Immunofluorescence (IF)
(IC/IF analysis of cytoplasmic expression of Cre Recombinase in exogenously transfected NIH/3T3 and HeLa cells using MBS603247 and a fluorescently conjugated anti-mouse secondary antibody (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nucleus was stained with DAPI (Blue).)
Mouse Cre Recombinase Monoclonal Antibody
Cre Recombinase
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
AscitesAscites
Synonyms
Cre Recombinase; Monoclonal Antibody; Anti -Cre Recombinase; anti-Cre Recombinase antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2Q2151
Specificity
Recognizes both native and recombinant Cre recombinase at ~38kD from all species.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid. No preservatives added.
Applicable Applications for anti-Cre Recombinase antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry.
Dilution: ELISA: 1:500-1:5000
Western Blot (Native & recombinant protein): 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Dilution: ELISA: 1:500-1:5000
Western Blot (Native & recombinant protein): 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Immunogen
Cre recombinase aa77-343 fusion protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(IC/IF analysis of cytoplasmic expression of Cre Recombinase in exogenously transfected NIH/3T3 and HeLa cells using MBS603247 and a fluorescently conjugated anti-mouse secondary antibody (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nucleus was stained with DAPI (Blue).)
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(IC/IF analysis of cytoplasmic expression of Cre Recombinase in exogenously transfected NIH/3T3 and HeLa cells using MBS603247 and a fluorescently conjugated anti-mouse secondary antibody (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nucleus was stained with DAPI (Blue).)
Related Product Information for anti-Cre Recombinase antibody
Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is a homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.
Product Categories/Family for anti-Cre Recombinase antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
cre recombinase
Similar Products
Product Notes
The Cre Recombinase (Catalog #AAA603247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cre Recombinase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Dilution: ELISA: 1:500-1:5000 Western Blot (Native & recombinant protein): 1:500-1:5000 Immunohistochemistry: 1:500-1:5000. Researchers should empirically determine the suitability of the Cre Recombinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cre Recombinase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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